Related Experiment Video
Updated: Jan 15, 2026

Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues
Published on: June 21, 2018
Coated blade spray-mass spectrometry for rapid screening: A tutorial
1Department of Chemistry, University of Waterloo, Waterloo, ON, N2L 3G1, Canada.
Abstract:
Coated blade spray-mass spectrometry (CBS-MS) integrates solid-phase microextraction (SPME) with ambient mass spectrometry (AMS), providing a powerful approach for analyzing trace analytes in complex sample matrices. In CBS-MS, analytes are extracted and enriched into a thin layer of coating material on a stainless-steel blade. Afterwards, a small volume of solvent is applied directly to the coating surface, enabling rapid desorption and subsequent electrospray ionization (ESI) from the blade tip under high voltage. By eliminating chromatographic separation and utilizing high-throughput extraction formats, CBS-MS enables rapid screening with total analysis times as short as 10 s per sample. The use of matrix-compatible coating materials facilitates direct analysis of complex biological matrices-including plasma, urine, whole blood, and tissue-without additional cleanup steps. This tutorial highlights the key features of CBS-MS, provides detailed guidance for its implementation, and offers perspectives on future developments of this emerging technology.
More Related Videos
09:49Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection
Published on: November 18, 2022
09:04Identifying Per- and Polyfluorinated Chemical Species with a Combined Targeted and Non-Targeted-Screening High-Resolution Mass Spectrometry Workflow
Published on: April 18, 2019
Related Concept Videos
MALDI-TOF Mass Spectrometry
Tandem Mass Spectrometry
Electrospray Ionization (ESI) Mass Spectrometry
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
Mass Spectrometry: Complex Analysis
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview
Mass Spectrometers