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Preparation of Model Intercellular Membrane Using Low-Toxic Solvent Ethanol.

Masayuki Iino1, Hazuki Iijima1, Ryuta Okada1

  • 1Research and Development Department, ALBION CO., LTD., 2-24-11 Higashi-nihombashi, Chuo-ku, Tokyo 103-0004, Japan.

Chemical & Pharmaceutical Bulletin
|October 15, 2025
PubMed
Summary

Researchers developed a new, safer ethanol-based method to create model skin barrier membranes. This approach uses less toxic solvents and yields comparable results to traditional toxic methods, improving environmental hygiene in skin research.

Keywords:
X-ray diffractioncalorimetryintercellular lamellastratum corneumstructurethermal phase behavior

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Area of Science:

  • Biochemistry
  • Materials Science
  • Dermatology

Background:

  • The skin's outermost layer, the stratum corneum, relies on intercellular lipids (IC lipids) like ceramides, cholesterol, and free fatty acids for barrier function.
  • Model membranes of these lipids are crucial for studying skin barrier mechanisms.
  • Conventional methods use toxic solvents (chloroform, methanol), raising safety and environmental concerns.

Purpose of the Study:

  • To develop a safer, environmentally friendly protocol for preparing model intercellular lipid membranes.
  • To evaluate the efficacy and comparability of the new protocol against traditional methods.

Main Methods:

  • A novel protocol using ethanol (EtOH) to dissolve and mix IC lipids.
  • Dilution with buffer and controlled cooling to form lamellar structures.
  • Collection of model membranes via centrifugation for analysis.

Main Results:

  • The ethanol-based protocol successfully prepared model intercellular lamellae.
  • The resulting membranes exhibited thermodynamic and structural properties comparable to those made with chloroform/methanol.
  • The EtOH protocol showed enhanced miscibility of IC lipids.

Conclusions:

  • The developed ethanol-based protocol offers a safer and more environmentally hygienic alternative for preparing model skin barrier membranes.
  • This methodology is suitable for research requiring model intercellular lamellae, aligning with green chemistry principles.