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Area of Science:

  • Spatial biology
  • Mass spectrometry imaging
  • Cellular analysis

Background:

  • Spatial biology analyzes cells in their microenvironment, including morphology and molecular expression.
  • Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) provides spatial lipid and metabolic profiles.
  • Integrating MALDI-MSI with other methods at the single-cell level is challenging due to co-registration and resolution issues.

Purpose of the Study:

  • To develop a MALDI-MSI based method for integrated spatial analysis.
  • To enable coupled (sub-)cellular investigation of the same sample using multiple modalities.
  • To combine lipid profiling with morphological and protein expression data at the single-cell level.

Main Methods:

  • Developed a MALDI-MSI method integrating in-source bright-field and fluorescence microscopy.
  • Applied the method to cell culture and tissue samples.
  • Enabled coupled analysis of lipid profiles and cellular features.

Main Results:

  • Visualized intracellular lipid distributions in macrophages during phagocytosis.
  • Revealed heterogeneity of lipid profiles in tumor-infiltrating neutrophils.
  • Correlated lipid profiles with individual microenvironments.

Conclusions:

  • The developed method allows for coupled (sub-)cellular investigation of lipid profiles and morphology.
  • This approach provides a powerful tool for single-cell level analysis in cell biology.
  • Enables deeper understanding of cellular functions within their native microenvironments.