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Updated: Jan 15, 2026

Characterization of Adipocyte-Derived Extracellular Vesicle Secretion Using a CD63-GFP Reporter Mouse Model In Vivo and In Vitro
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When Fat Talks: How Adipose-Derived Extracellular Vesicles Fuel Breast Cancer.

Maria Pia Cavaleri1, Tommaso Pusceddu1, Lucia Sileo1

  • 1Translational Medicine Department, University of Ferrara, 44121 Ferrara, Italy.

International Journal of Molecular Sciences
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Summary

Adipose tissue

Keywords:
adipocytebreast cancer cellsexosomeextracellular vesicles

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Area of Science:

  • Oncology
  • Cell Biology
  • Biochemistry

Background:

  • Adipose tissue is a key component of the tumor microenvironment (TME).
  • Adipocyte-secreted extracellular vesicles (EVs) mediate intercellular communication within the TME.
  • The precise mechanisms of adipocyte-EV influence on breast cancer (BC) progression require elucidation.

Purpose of the Study:

  • To investigate the role of adipocyte-derived EVs in breast cancer progression.
  • To understand the bidirectional signaling between adipocytes and breast cancer cells via EVs.

Main Methods:

  • Human mesenchymal stem cells (hMSCs) differentiated into adipocytes.
  • Isolation of EVs from adipocyte and breast cancer cell conditioned media.
  • Characterization of EVs using nanoparticle tracking analysis and transmission electron microscopy.
  • Assessment of EV uptake and functional effects on recipient cells via fluorescence microscopy and gene expression analysis.

Main Results:

  • Breast cancer cell-derived EVs reduced stromal cell viability and promoted apoptosis resistance.
  • Adipocyte-derived EVs altered breast cancer cell behavior, indicating bidirectional communication.
  • EVs facilitate a dynamic exchange of bioactive signals between tumor and stromal compartments.

Conclusions:

  • EV-mediated communication is crucial for tumor-stroma interactions in breast cancer.
  • Adipocyte-cancer cell EV crosstalk contributes to TME remodeling.
  • This crosstalk may facilitate breast cancer progression and presents potential therapeutic targets.