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Updated: Jan 15, 2026
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Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics
Published on: June 23, 2022
2.9K
Proteomics Insights Into Lysosome Biogenesis and Maturation.
Katharina Hirn1, Sofía Fajardo-Callejón1,2, Dominic Winter1,2
1Department Metabolism, Senescence and Autophagy, Research Center One Health Ruhr, Medical Faculty, University Alliance Ruhr, University Hospital Essen, University of Duisburg-Essen, Essen, Germany.
Proteomics
|October 16, 2025
Summary
Lysosomes are key cellular recycling centers. Mass spectrometry proteomics has significantly advanced our understanding of lysosome biogenesis and function over the past 20 years, identifying over 350 crucial proteins.
Area of Science:
- Cell Biology
- Biochemistry
- Proteomics
Background:
- Lysosomes are vital degradative organelles in eukaryotic cells, essential for cellular homeostasis and metabolic signaling.
- Lysosomal dysfunction is linked to various human diseases, underscoring their importance.
- Lysosomal biogenesis is a complex, multi-step process requiring precise control of hydrolase activity and membrane assembly.
Purpose of the Study:
- To review the impact of mass spectrometry (MS)-based proteomics on characterizing lysosomal biogenesis over the last two decades.
- To highlight the role of proteomic analysis in understanding lysosome function and protein identification.
Main Methods:
- Enrichment and proteomic analysis of lysosomes and lysosomal proteins.
- Mass spectrometry (MS)-based proteomics techniques.
Main Results:
- Proteomics has been instrumental in investigating lysosomes, including gene expression control, sorting, and trafficking.
- Over the past 20 years, MS-based proteomics has identified approximately 350 proteins localized to or critical for lysosome function.
- This has facilitated a deeper understanding of the molecular machinery governing lysosomal biogenesis.
Conclusions:
- Mass spectrometry proteomics is a powerful tool for dissecting complex cellular processes like lysosomal biogenesis.
- Continued proteomic studies will further elucidate lysosome biology and its role in health and disease.
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