Related Experiment Video
Updated: Jan 15, 2026
Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
[Multi-omics association among intestinal microbiome, metabolome, and histone H4 acetylation in children with asthma]
Ping Zheng1, Chuanchuan Yu1, Xifang Lyu1
1National Institute of Environmental Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Objective:
To analyze the correlation between intestinal microbiome, metabolome and histone H4 acetylation level in children with asthma, and explore the mechanism that gut microbiome affects children with asthma.
Methods:
Histone H4 acetylation in induced sputum, 16S rRNA gene sequencing of gut microbiome, and untargeted fecal metabolomics were detected in asthmatic children and control children. Network method was used to analyze the correlation between characteristic gut microbes of asthma and histone H4 acetylation, Quantile g-computation(QGC)was used to analyze the effect of characteristic microbes on histone H4 acetylation, and the mediation model was used to analyze the mediating effect of metabolites in the process of gut microbes affecting histone H4 acetylation.
Results:
The mean A(optical density) value of histone H4 acetylation in sputum was 0.668±0.260 in asthmatic children(n=37), and 0.593±0.069 in control group(n=20). A total of 5 characteristic gut genera of children with asthma were significantly correlated with H4 acetylation level(adjusted P<0.05). QGC analysis showed that the 5 characteristic gut genera had a positive effect on the histone acetylation level of histone H4(P=0.001), and the A value of histone H4 acetylation increased by 0.124 for every 1 quantile increase in the relative abundance of characteristic gut genera(95%CI 0.058-0.190). By spearman analysis, 34 fecal metabolites were significantly correlated with the five characteristic gut genera and histone H4 acetylation levels. Prevotella was found have influenced the level of H4 acetylation through the mediating effect of N-delta-acetylornithine, phenethylamine, 2-hydroxy-3-methylvalerate, alpha-ketoglutaramate, gamma-glutamylglycine, and mevalonolactone.
Conclusion:
The metabolites related with the characteristic gut bacteria in asthmatic children were associated with histone H4 acetylation, which suggested that the gut microbiome may induce childhood asthma by regulating epigenetic variation.
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