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Cold Plasma Pretreatment Enhances Antioxidant Stability in Lime Fruit During Storage
Ghazal Razazian1, Mohsen Dalvi-Isfahan1, Saeideh Mohtashami2
1Department of Food Science and Technology, Faculty of Agriculture Jahrom University Jahrom Fars Iran.
Food Science & Nutrition
|October 16, 2025
Summary
Cold plasma (CP) treatment, especially dielectric barrier discharge (DBD), enhances antioxidant compounds and extends the shelf life of limes. This nonchemical method improves nutritional value and postharvest stability in citrus fruits.
Area of Science:
- Food Science
- Postharvest Technology
- Plant Physiology
Background:
- Limes (Citrus aurantiifolia) are rich in antioxidants but have a short shelf life due to deterioration.
- Nonchemical methods are sought to improve fruit quality and longevity.
Purpose of the Study:
- To investigate the impact of cold plasma (CP) pretreatment on the antioxidant properties and storage stability of limes.
- To compare the efficacy of dielectric barrier discharge (DBD) and gliding arc (GLIDE) plasma.
Main Methods:
- Limes were treated with DBD and GLIDE cold plasma.
- Analysis included quantification of bioactive compounds (Vitamin C, phenolics, flavonoids, tannins).
- Surface analysis used Fourier-transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM).
Main Results:
- CP treatment significantly increased key bioactive compounds, particularly in the peel.
- DBD plasma at 10 minutes showed the most significant enhancement of antioxidants.
- FTIR and SEM revealed CP-induced surface modifications affecting enzymatic activity.
- Antioxidant activity showed an initial decrease followed by a recovery phase in CP-treated limes.
Conclusions:
- Cold plasma, especially DBD, effectively enhances the antioxidant profile of limes.
- CP treatment improves postharvest stability and nutritional properties of citrus fruits.
- CP offers a promising nonchemical alternative for fruit preservation and quality enhancement.

