Workflow for efficiently isolating microspore cultures of different rice genotypes by optimizing the callus induction...

Area of Science:

• Plant Science
• Agricultural Biotechnology
• Genetics

Background:

• Rice is a vital global food source, crucial for food security.
• Doubled haploid technology accelerates rice breeding but faces challenges like genotypic dependency and low efficiency.
• Isolated microspore culture offers a potential solution to improve breeding timelines.

Purpose of the Study:

• To optimize isolated microspore culture for diverse rice genotypes.
• To identify optimal callus induction media and carbon sources for rice microspore culture.
• To evaluate plant regeneration efficiency and spontaneous doubling rates.

Main Methods:

• Screened 15 rice genotypes (12 japonica, 3 indica) for microspore culture.
• Compared effects of different callus induction media (CIMs) and carbon sources.
• Assessed plant regeneration and ploidy levels of regenerated seedlings.

Main Results:

• Maltose identified as the optimal carbon source.
• CIM III demonstrated superior performance for callus induction and yield.
• 12 of 14 genotypes regenerated green seedlings, primarily japonica.
• Spontaneous doubling rates ranged from 14.3% to 98%, exceeding anther culture rates.

Conclusions:

• An effective isolated microspore culture method was established for various rice genotypes.
• This method enhances doubled haploid technology application in rice breeding.
• Provides improved options for accelerating rice crop improvement and ensuring food security.