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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Designing a Bio-responsive Robot from DNA Origami
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Nanoscale Precise Stamping of Biomolecule Patterns Using DNA Origami.

Laura Teodori1,2,3, Ali Shahrokhtash1,2, Elisabeth A Sørensen1,2

  • 1Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Gustav Wieds Vej 14, 8000 Aarhus C, Denmark.

ACS Nano
|October 16, 2025
PubMed
Summary
This summary is machine-generated.

This study introduces a rapid DNA origami stamping method for precise nanoscale surface patterning. The technique enables efficient and accurate transfer of oligonucleotide patterns, advancing studies of distance-dependent biological processes.

Keywords:
DNA origamiDNA-PAINTbiopatterningsingle-moleculesurface functionalization

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Area of Science:

  • Nanotechnology
  • Molecular Biology
  • Biophysics

Background:

  • Precise control over molecular positioning is crucial for understanding biological processes.
  • DNA origami offers nanoscale precision but faces limitations in stability and scalability for biological applications.
  • Existing surface patterning methods often require specialized equipment and lack accessibility.

Purpose of the Study:

  • To develop a straightforward and rapid DNA origami stamping technique for transferring nanoscale oligonucleotide patterns onto surfaces.
  • To quantitatively assess stamping efficiency and precision using DNA-PAINT super-resolution microscopy.
  • To provide an accessible, self-assembled platform for versatile surface patterning applicable to various substrates.

Main Methods:

  • Developed a DNA origami stamping technique for pattern transfer.
  • Utilized DNA-PAINT super-resolution microscopy for visualization and quantitative assessment.
  • Employed modifiable pattern-transfer oligonucleotides for substrate versatility.
  • Incorporated passivated surfaces to limit nonspecific interactions.

Main Results:

  • Demonstrated reliable, efficient, and precise pattern transfer at single-molecule resolution.
  • Showcased versatility across different stamp types and substrates.
  • Validated the technique's ability to control interactions between biological targets and patterned biomolecules.

Conclusions:

  • The DNA origami stamping technique offers an accessible and cost-effective approach to nanoscale surface patterning.
  • This method facilitates the study of distance-dependent biological phenomena, such as receptor activation and multivalent binding.
  • The combination of DNA nanotechnology and single-molecule imaging expands analytical capabilities and enables multiplexed detection and live measurements.