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Nanoscale Precise Stamping of Biomolecule Patterns Using DNA Origami
Laura Teodori1,2,3, Ali Shahrokhtash1,2, Elisabeth A Sørensen1,2
1Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Gustav Wieds Vej 14, 8000 Aarhus C, Denmark.
ACS Nano
|October 16, 2025
Summary
This study introduces a rapid DNA origami stamping method for precise nanoscale surface patterning. The technique enables efficient and accurate transfer of oligonucleotide patterns, advancing studies of distance-dependent biological processes.
Area of Science:
- Nanotechnology
- Molecular Biology
- Biophysics
Background:
- Precise control over molecular positioning is crucial for understanding biological processes.
- DNA origami offers nanoscale precision but faces limitations in stability and scalability for biological applications.
- Existing surface patterning methods often require specialized equipment and lack accessibility.
Purpose of the Study:
- To develop a straightforward and rapid DNA origami stamping technique for transferring nanoscale oligonucleotide patterns onto surfaces.
- To quantitatively assess stamping efficiency and precision using DNA-PAINT super-resolution microscopy.
- To provide an accessible, self-assembled platform for versatile surface patterning applicable to various substrates.
Main Methods:
- Developed a DNA origami stamping technique for pattern transfer.
- Utilized DNA-PAINT super-resolution microscopy for visualization and quantitative assessment.
- Employed modifiable pattern-transfer oligonucleotides for substrate versatility.
- Incorporated passivated surfaces to limit nonspecific interactions.
Main Results:
- Demonstrated reliable, efficient, and precise pattern transfer at single-molecule resolution.
- Showcased versatility across different stamp types and substrates.
- Validated the technique's ability to control interactions between biological targets and patterned biomolecules.
Conclusions:
- The DNA origami stamping technique offers an accessible and cost-effective approach to nanoscale surface patterning.
- This method facilitates the study of distance-dependent biological phenomena, such as receptor activation and multivalent binding.
- The combination of DNA nanotechnology and single-molecule imaging expands analytical capabilities and enables multiplexed detection and live measurements.
