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Updated: Jan 15, 2026

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CircLSM14A regulates PEDV replication via the miR-27b-5p/HMGB1 axis

Yuxuan Wang1, Bin Liu1, Jiaqi Liang2

  • 1College of Animal Science, Guangdong Province Key Laboratory of Animal Nutritional Regulation, National Engineering Research Center for Breeding Swine Industry, State Key Laboratory of Livestock and Poultry Breeding, South China Agricultural University, Guangzhou, Guangdong 510642, China.

Veterinary Microbiology
|October 16, 2025
PubMed

Insights

Porcine milk exosomes contain circLSM14A, which enhances porcine epidemic diarrhea virus (PEDV) replication by sponging miR-27b-5p. This study reveals a novel regulatory mechanism for PEDV infection in piglets.

Area of Science:

  • Veterinary Virology
  • Molecular Biology
  • Exosome Biology

Background:

  • Circular RNAs (circRNAs) are key regulators in host-virus interactions.
  • The role of porcine milk exosome-derived circRNAs in porcine epidemic diarrhea virus (PEDV) replication is largely unknown.

Purpose of the Study:

  • To investigate the regulatory mechanism of porcine milk exosome-derived circRNAs on PEDV replication in piglets.
  • To identify specific circRNAs involved in this process.

Main Methods:

  • Sequencing analysis of non-coding RNAs in porcine milk exosomes.
  • Validation in cell culture and piglet intestinal organoid models.
  • Molecular assays to determine molecular interactions and signaling pathways.

Main Results:

  • circLSM14A was identified in porcine milk exosomes and acts as a sponge for miR-27b-5p.
  • circLSM14A inhibition of miR-27b-5p led to increased HMGB1 expression.
  • Upregulation of downstream molecules (TLR4, NF-κB, Beclin1) by HMGB1 promoted PEDV replication.

Conclusions:

  • circLSM14A in milk exosomes is a novel regulator promoting PEDV replication.
  • This mechanism involves the circLSM14A/miR-27b-5p/HMGB1 axis.
  • Findings offer new strategies for PEDV prevention and control.
Keywords:
CircLSM14AHMGB1PEDVViral replicationmiR-27b-5p

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