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A protein FAR-RED ELONGATED HYPOCOTYL 1 played a positive role in plant height modulation in ornamental kale
Can Huo1, Yang Liu1, Xin Feng2
1College of Forestry, Shenyang Agricultural University, Shenyang, 110866, China.
Abstract:
Height is an important horticultural trait that affects the ornamental value of kale. At present, the genetic control of the plant height development in kale remains unclear. Here, we identified the BoFHY1 gene, encoding a protein FAR-RED ELONGATED HYPOCOTYL 1, based on our previous BSA-seq genetic data from a tall cut-flower inbred line and a dwarf ground-cover line. Sequence alignment revealed three nonsynonymous single nucleotide polymorphisms in the coding sequence of BoFHY1 between the tall and dwarf materials. The spatiotemporal transcription pattern of BoFHY1 showed that it was most highly expressed in the hypocotyl and the stem apex at 5-leaf stage. Furthermore, we verified the biological function of BoFHY1 by generating overexpression and CRISPR/Cas9 knockout lines. The overexpression lines possessed elongated internodes and height, whereas the mutant Bofhy1 exhibited shorter internodes and plant height compared to their respective wild-type. These data suggested that BoFHY1 played a positive role in regulating plant height of kale. Moreover, Y2H and BiFC assays showed that there was no direct interaction between BoFHY1 and BoFHL, another phyA partner. This indicated that BoFHY1 might function independently of BoFHL in transporting phyA. Our results provided valuable information for understanding the genetic regulation of plant height in kale and enriched the gene resources available for plant height improvement.
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