Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

New obligate methylotroph.

J S Dahl, R J Mehta, D S Hoare

    Journal of Bacteriology
    |February 1, 1972
    PubMed
    Summary
    This summary is machine-generated.

    A novel obligate methylotroph utilizes only methanol and methylamine for growth, lacking complex membranes and key tricarboxylic acid cycle enzymes. This unique organism employs the ribose phosphate cycle for methyl metabolism.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Hemodynamic Response to Rapid Saline Infusion Compared with Exercise in Healthy Participants Aged 20-80 Years.

    Journal of cardiac failure·2019
    Same author

    Odor control during post-digestion processing of biosolids through bioaugmentation of anaerobic digestion.

    Water science and technology : a journal of the International Association on Water Pollution Research·2008
    Same author

    Transmembrane-truncated alphavbeta3 integrin retains high affinity for ligand binding: evidence for an 'inside-out' suppressor?

    The Biochemical journal·1998
    Same author

    Structural and functional characterization and cloning of Xenopus FOSP-1 (frog oviduct-specific protein-1) genes.

    European journal of biochemistry·1995
    Same author

    Fungitoxicity of m-fluorophenylalanine-containing peptides towards Pythium ultimum.

    Experientia·1989
    Same author

    Actinoidin A2, a novel glycopeptide: production, preparative HPLC separation and characterization.

    The Journal of antibiotics·1987
    Same journal

    The bacterial SOS response promotes the expression of the transposase encoded by IS<i>CR</i> mobile genetic elements.

    Journal of bacteriology·2026
    Same journal

    Development of a gene-editing strategy to overcome genetic intractability in <i>Lactobacillus johnsonii</i>.

    Journal of bacteriology·2026
    Same journal

    Bactofilins are essential spatial organizers of peptidoglycan insertion in the Lyme disease spirochete <i>Borrelia burgdorferi</i>.

    Journal of bacteriology·2026
    Same journal

    DNA damage-associated vesicle production in <i>Stenotrophomonas maltophilia</i> is mediated by the maltocin endolysin.

    Journal of bacteriology·2026
    Same journal

    Characterization of <i>Helicobacter pylori</i> aggregation reveals a requirement for both AlpA and AlpB.

    Journal of bacteriology·2026
    Same journal

    Cross-regulation of amino acid synthesis and anaerobic electron transfer by MetR-mediated methionine signaling.

    Journal of bacteriology·2026
    See all related articles

    Area of Science:

    • Microbiology
    • Biochemistry
    • Methylotrophy

    Background:

    • Methylotrophs are microorganisms capable of utilizing one-carbon compounds.
    • Obligate methylotrophs exclusively use methylated compounds for energy and carbon.
    • Understanding methylotroph metabolism is crucial for biotechnology and environmental science.

    Purpose of the Study:

    • To isolate and characterize a novel obligate methylotroph.
    • To elucidate the metabolic pathways of this unique organism.
    • To compare its characteristics with a facultative methylotroph.

    Main Methods:

    • Isolation of methylotrophs from enrichment cultures using methanol.
    • Growth studies on various one-carbon compounds (methanol, methylamine, methane).

    Related Experiment Videos

  • Analysis of intracellular membrane systems and enzyme activities (tricarboxylic acid cycle, hexose phosphate synthetase).
  • (14)C-acetate assimilation studies.

    • Main Results:

    • A unique obligate methylotroph was isolated, growing solely on methanol and methylamine, not methane.
    • The organism lacks a complex intracellular membrane system.
    • (14)C-acetate assimilation was observed but limited to specific cell constituents and dependent on methanol/methylamine oxidation.
    • Low levels of tricarboxylic acid cycle enzymes were detected; alpha-ketoglutarate dehydrogenase was absent.
    • Hexose phosphate synthetase, a key enzyme in the ribulose monophosphate pathway, was present.

    Conclusions:

    • The newly isolated obligate methylotroph possesses a distinct metabolic strategy.
    • Its metabolism relies on the ribose phosphate cycle, not the complete tricarboxylic acid cycle.
    • This finding expands the known diversity of methylotrophic organisms and their biochemical pathways.