Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genome-wide association mapping and targeted loss of function studies identify <i>Shroom3</i> as a driver of hyperpolyploidy and ventricular dilation.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Affinity-maturation engineering via phage display to optimize anti-idiotype antibodies for neutralizing antibody assays.

Bioanalysis·2026
Same author

Assessment of Immune Responses Against AAV Encoded Transgene Products.

The AAPS journal·2026
Same author

Implementing Synthetic Multi-Transmembrane Receptor Protein in a Duplex Immunoassay for Assessment of Neutralizing Antibody to a Bispecific Biotherapeutic.

The AAPS journal·2025
Same author

Understanding the role of Shroom3 in the developing mouse myocardium.

PloS one·2025
Same author

Assessment of Neutralizing Antibody Activity in Clinical Studies: Use of Surrogate Measurements Instead of Stand-alone Assays.

The AAPS journal·2025

Related Experiment Video

Updated: Jan 14, 2026

Optogenetic Manipulation of Neuronal Activity to Modulate Behavior in Freely Moving Mice
14:40

Optogenetic Manipulation of Neuronal Activity to Modulate Behavior in Freely Moving Mice

Published on: October 27, 2020

19.8K

Mapping Shroom3 expression across the adult mouse.

Jennifer L Carleton1, Dewmini Dematagoda1, Meg A Schuurman1

  • 1Children's Health Research Institute, Victoria Research Labs, 790 Commissioners Road East, London, Ontario, N6A 4L6, Canada; Department of Physiology & Pharmacology, The University of Western Ontario, 1151 Richmond Street, London, Ontario, N6A 3K7, Canada.

Gene Expression Patterns : GEP
|October 16, 2025
PubMed
Summary

Shroom3 protein, vital for development, is newly found in adult mouse bladder and reproductive systems. This discovery expands understanding of Shroom3

Keywords:
Gene expressionImmunofluorescenceLacZ reporter geneProtein expressionShroom3

More Related Videos

Dissection and Immunofluorescent Staining of Mushroom Body and Photoreceptor Neurons in Adult Drosophila melanogaster Brains
10:13

Dissection and Immunofluorescent Staining of Mushroom Body and Photoreceptor Neurons in Adult Drosophila melanogaster Brains

Published on: November 6, 2017

20.5K
Stereotaxic Surgery for Genetic Manipulation in Striatal Cells of Neonatal Mouse Brains
09:44

Stereotaxic Surgery for Genetic Manipulation in Striatal Cells of Neonatal Mouse Brains

Published on: July 10, 2018

16.2K

Related Experiment Videos

Last Updated: Jan 14, 2026

Optogenetic Manipulation of Neuronal Activity to Modulate Behavior in Freely Moving Mice
14:40

Optogenetic Manipulation of Neuronal Activity to Modulate Behavior in Freely Moving Mice

Published on: October 27, 2020

19.8K
Dissection and Immunofluorescent Staining of Mushroom Body and Photoreceptor Neurons in Adult Drosophila melanogaster Brains
10:13

Dissection and Immunofluorescent Staining of Mushroom Body and Photoreceptor Neurons in Adult Drosophila melanogaster Brains

Published on: November 6, 2017

20.5K
Stereotaxic Surgery for Genetic Manipulation in Striatal Cells of Neonatal Mouse Brains
09:44

Stereotaxic Surgery for Genetic Manipulation in Striatal Cells of Neonatal Mouse Brains

Published on: July 10, 2018

16.2K

Area of Science:

  • Cell Biology
  • Developmental Biology
  • Genetics

Background:

  • Shroom3 is an actin-binding protein crucial for mammalian morphogenesis, involved in apical constriction and elongation.
  • Mutations in Shroom3 are linked to human congenital defects like anencephaly and spina bifida.
  • Genome-wide association studies suggest Shroom3's role in adult conditions, including chronic kidney disease and heterotaxy.

Purpose of the Study:

  • To investigate the unexplored adult expression patterns of Shroom3.
  • To characterize the localization of Shroom3 protein within adult tissues.
  • To identify tissues with previously unknown Shroom3 expression.

Main Methods:

  • Utilized adult mice heterozygous for a Shroom3 LacZ reporter (Shroom3+/Gt).
  • Performed wholemount X-gal staining and glycerol clearing to map Shroom3 expression.
  • Employed fluorescent staining on wildtype organs to localize Shroom3 protein.

Main Results:

  • Identified novel Shroom3 expression in adult mouse bladder and reproductive systems.
  • Confirmed postnatal Shroom3 expression in organs like the eye and whisker pad.
  • Observed no Shroom3 expression in adult liver and pancreas.

Conclusions:

  • Shroom3 exhibits a broader expression pattern in adult mammals than previously recognized.
  • The findings suggest potential roles for Shroom3 in adult tissue biology and pathology.
  • This study provides a basis for future research into Shroom3's functions in diverse tissues.