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Mapping Shroom3 expression across the adult mouse
Jennifer L Carleton1, Dewmini Dematagoda1, Meg A Schuurman1
1Children's Health Research Institute, Victoria Research Labs, 790 Commissioners Road East, London, Ontario, N6A 4L6, Canada; Department of Physiology & Pharmacology, The University of Western Ontario, 1151 Richmond Street, London, Ontario, N6A 3K7, Canada.
Gene Expression Patterns : GEP
|October 16, 2025
Summary
Shroom3 protein, vital for development, is newly found in adult mouse bladder and reproductive systems. This discovery expands understanding of Shroom3
Area of Science:
- Cell Biology
- Developmental Biology
- Genetics
Background:
- Shroom3 is an actin-binding protein crucial for mammalian morphogenesis, involved in apical constriction and elongation.
- Mutations in Shroom3 are linked to human congenital defects like anencephaly and spina bifida.
- Genome-wide association studies suggest Shroom3's role in adult conditions, including chronic kidney disease and heterotaxy.
Purpose of the Study:
- To investigate the unexplored adult expression patterns of Shroom3.
- To characterize the localization of Shroom3 protein within adult tissues.
- To identify tissues with previously unknown Shroom3 expression.
Main Methods:
- Utilized adult mice heterozygous for a Shroom3 LacZ reporter (Shroom3+/Gt).
- Performed wholemount X-gal staining and glycerol clearing to map Shroom3 expression.
- Employed fluorescent staining on wildtype organs to localize Shroom3 protein.
Main Results:
- Identified novel Shroom3 expression in adult mouse bladder and reproductive systems.
- Confirmed postnatal Shroom3 expression in organs like the eye and whisker pad.
- Observed no Shroom3 expression in adult liver and pancreas.
Conclusions:
- Shroom3 exhibits a broader expression pattern in adult mammals than previously recognized.
- The findings suggest potential roles for Shroom3 in adult tissue biology and pathology.
- This study provides a basis for future research into Shroom3's functions in diverse tissues.
