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Kaempferol regulates Ewing sarcoma progression via miR-26b-5p-mediated expression of the family with sequence

Yiquan Ji1, Tian Gao1, Zehua Xu1

  • 1Department of Orthopaedics, The Second Hospital of Jiaxing, No. 1518, Huancheng Road Nanhu District, Jiaxing, Zhejiang Province, 314000, P.R. China.

BMC Pharmacology & Toxicology
|October 17, 2025
PubMed

Insights

Kaempferol, a natural flavonoid, effectively inhibits Ewing sarcoma (ES) progression by upregulating miR-26b-5p, suppressing FAM98A, and blocking key cancer pathways. This natural compound shows promise for treating this aggressive pediatric bone cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • Ewing sarcoma (ES) is an aggressive pediatric bone cancer with poor prognosis for metastatic or recurrent disease.
  • Current treatment options for advanced ES are limited, necessitating the exploration of novel therapeutic strategies.

Purpose of the Study:

  • To investigate the antitumor effects of kaempferol, a natural flavonoid, on Ewing sarcoma.
  • To elucidate the molecular mechanisms underlying kaempferol's anti-ES activity.

Main Methods:

  • In vitro studies using SK-ES-1 cells to assess proliferation, migration, and invasion.
  • Molecular analysis including miR-26b-5p and FAM98A expression, and Western blotting for signaling pathway components.
  • In vivo xenograft mouse model to evaluate kaempferol's efficacy on tumor growth.

Main Results:

  • Kaempferol significantly inhibited ES cell proliferation, migration, and invasion in a dose- and time-dependent manner.
  • Kaempferol upregulated miR-26b-5p, which suppressed FAM98A expression, leading to inhibition of the EGFR/PI3K/AKT/NF-κB pathway.
  • Kaempferol suppressed tumor growth in vivo, an effect partially reversed by miR-26b-5p knockdown.

Conclusions:

  • Kaempferol demonstrates significant antitumor effects against Ewing sarcoma.
  • The mechanism involves modulation of the miR-26b-5p/FAM98A axis and downstream signaling pathways.
  • Kaempferol represents a potential novel therapeutic agent for Ewing sarcoma treatment.
Keywords:
EGFR/PI3K/AKT/NF-κB pathwayEwing sarcomaFAM98AKaempferolmiR-26b-5p

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