Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Combined Omics Approach to Elucidate the Molecular Interplay behind a Beneficial <i>Arabidopsis-Caulobacter</i> Interaction.

Journal of proteome research·2026
Same author

Author Correction: Community benchmarking and evaluation of human unannotated microprotein detection by mass spectrometry based proteomics.

Nature communications·2026
Same author

Single-cell proteomics reveals cytoplasmic defects in Patl2-deficient oocytes rescued by spindle transfer.

Human reproduction (Oxford, England)·2026
Same author

Advancing DIA-Based Limited Proteolysis Workflows: Introducing DIA-LiPA.

Analytical chemistry·2026
Same author

From alkylating to shape-shifting G-quadruplex ligands: the RHAU peptide story.

Nucleic acids research·2026
Same author

Community benchmarking and evaluation of human unannotated microprotein detection by mass spectrometry based proteomics.

Nature communications·2026
Same journal

Proteomic Profiling of Endothelial Cells Under Laminar Shear Stress Confirms the Importance of KLF4 in the Regulation of Membrane Protein Expression Compared to Oscillatory Flow.

Journal of proteome research·2026
Same journal

Identification of Age-Associated Circulating Proteins and Lipids in 3800 Comorbidity-Enriched Older Adults from Japan-Based Cohorts Using Olink Assays and MRM Mass Spectrometry.

Journal of proteome research·2026
Same journal

Molecular Solution to the Paradox of Ancient Brain Preservation.

Journal of proteome research·2026
Same journal

From Method-Defined Signals to Reference Measurement Procedures: Two Decades of Mass Spectrometry-Based ProGRP Quantification.

Journal of proteome research·2026
Same journal

Proteomic Profiling of Extracellular Vesicle-Enriched Plasma Using Mag-Net for Biomarker Discovery in Pancreatic Ductal Adenocarcinoma.

Journal of proteome research·2026
Same journal

Computationally Efficient Bayesian Estimation of Graphical Networks for Omics Data.

Journal of proteome research·2026
See all related articles

Related Experiment Video

Updated: Jan 14, 2026

Determining Cell-surface Expression and Endocytic Rate of Proteins in Primary Astrocyte Cultures Using Biotinylation
10:42

Determining Cell-surface Expression and Endocytic Rate of Proteins in Primary Astrocyte Cultures Using Biotinylation

Published on: July 3, 2017

10.0K

Profiling the Cellular Surfaceome by Furan-Based Protein Biotinylation.

Esperanza Fernández1,2, Laia Miret-Casals1,3, Annemieke Madder3

  • 1VIB Center for Medical Biotechnology, VIB, B-9052 Ghent, Belgium.

Journal of Proteome Research
|October 17, 2025
PubMed
Summary
This summary is machine-generated.

This study introduces a new furan chemistry method for profiling the cellular surfaceome. The novel furan-biotin compound FB1 efficiently and specifically labels cell surface proteins for proteomic analysis.

Keywords:
LC−MSMSaffinity purificationcellular surfaceomefuran-biotinplasma membrane proteinsproteomics

More Related Videos

"Cell Surface Capture" Workflow for Label-Free Quantification of the Cell Surface Proteome
06:31

"Cell Surface Capture" Workflow for Label-Free Quantification of the Cell Surface Proteome

Published on: March 24, 2023

3.0K
In Vivo Proximity Biotinylation for Protein Interaction Studies in Paramecium tetraurelia
06:43

In Vivo Proximity Biotinylation for Protein Interaction Studies in Paramecium tetraurelia

Published on: September 12, 2025

1.1K

Related Experiment Videos

Last Updated: Jan 14, 2026

Determining Cell-surface Expression and Endocytic Rate of Proteins in Primary Astrocyte Cultures Using Biotinylation
10:42

Determining Cell-surface Expression and Endocytic Rate of Proteins in Primary Astrocyte Cultures Using Biotinylation

Published on: July 3, 2017

10.0K
"Cell Surface Capture" Workflow for Label-Free Quantification of the Cell Surface Proteome
06:31

"Cell Surface Capture" Workflow for Label-Free Quantification of the Cell Surface Proteome

Published on: March 24, 2023

3.0K
In Vivo Proximity Biotinylation for Protein Interaction Studies in Paramecium tetraurelia
06:43

In Vivo Proximity Biotinylation for Protein Interaction Studies in Paramecium tetraurelia

Published on: September 12, 2025

1.1K

Area of Science:

  • Proteomics
  • Cell Biology
  • Chemical Biology

Background:

  • The cellular surfaceome is vital for cell function and communication.
  • Analyzing surface proteins is difficult due to their hydrophobic nature and low abundance, hindering mass spectrometry.
  • Existing methods often underrepresent surface proteins or suffer from nonspecific labeling.

Purpose of the Study:

  • To develop a novel, robust, and specific method for profiling the cellular surfaceome.
  • To overcome limitations of current proteomic techniques for surface protein analysis.
  • To enhance the unbiased and high-specificity investigation of cell surface proteins.

Main Methods:

  • Synthesis of six furan-biotin compounds (FB1-FB6).
  • Evaluation of FB1's reactivity with lysine and cysteine residues.
  • Assessment of FB1's staining intensity and specificity upon furan activation by oxidation.
  • Utilizing biotinylated protein pull-downs for enrichment of cell surface proteins.
  • Comparison with N-hydroxysuccinimide ester-based biotinylation methods.

Main Results:

  • FB1 demonstrated reactivity with lysine and cysteine.
  • FB1 exhibited superior staining intensity and specificity for cell surface proteins after oxidation.
  • Biotinylated protein pull-downs confirmed efficient enrichment of cell surface proteins.
  • FB1 significantly reduced nonspecific labeling of intracellular proteins compared to NHS ester methods.

Conclusions:

  • The developed furan chemistry-based method provides a powerful tool for surfaceome profiling.
  • FB1 enables unbiased and high-specificity proteomic studies of the cell surface.
  • This approach addresses key challenges in membrane protein analysis, improving surfaceome characterization.