Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Targeted Cancer Therapies02:57

Targeted Cancer Therapies

8.6K
The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
8.6K
Tail-anchoring of Proteins in the ER Membrane01:45

Tail-anchoring of Proteins in the ER Membrane

3.7K
Tail-anchored, or TA, proteins are estimated to make up to 3-5% of membrane proteins found in the eukaryotic cell. Such proteins have a single transmembrane domain located approximately 30 amino acid residues upstream from the C-terminal end. As a result, the signal recognition particle (SRP) cannot guide a TA protein to the ER membrane for cotranslational insertion. Hence, they are integrated into the ER membrane post-translationally using their C-terminal end as the anchor. TA proteins...
3.7K
Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

7.7K
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
7.7K
Directing Proteins to the Rough Endoplasmic Reticulum01:34

Directing Proteins to the Rough Endoplasmic Reticulum

16.8K
The organelle-specific signaling sequences direct proteins synthesized in the cytosol to their final destination like ER, mitochondria, peroxisomes, etc. Some of the proteins directed to ER are then trafficked via vesicles to other organelles within the cell or the extracellular environment through the Golgi complex. For example, the rough ER synthesizes soluble proteins for transportation to the lysosomes or secretion out of the cell. It can also synthesize transmembrane proteins that can...
16.8K
Transducer Mechanism: Enzyme-Linked Receptors01:27

Transducer Mechanism: Enzyme-Linked Receptors

3.9K
Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
Major types that are helpful drug targets include:
3.9K
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

2.8K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR...
2.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

NMR-Based Metabolic Profiling of Biobank Derived Blood Samples for the Identification of Liver Disease Biomarkers.

Biomolecules & therapeutics·2026
Same author

Pharmacomultiomics suggests new off-targets for targeted anti-cancer agents.

Journal of pharmaceutical analysis·2026
Same author

Efficacy of a mobile-based intervention for newly graduated nurses: A randomized controlled trial.

Digital health·2026
Same author

Photoreductive Degradation of Fe(III)-Catechol Crosslinked Hydrogels under Visible Light.

Chemistry, an Asian journal·2026
Same author

Root plasticity and xylem modifications drive drought resilience in okra [<i>Abelmoschus esculentus</i> (L.) Moench] at the seedling stage.

Frontiers in plant science·2025
Same author

DN200434: An Inverse Agonist of Estrogen Related Receptor Gamma Enhances Na+/I- symporter Function through Mitogen-Activated Protein Kinase Signaling in Radioiodine-Refractory Papillary Thyroid Cancer Cells.

Asian Pacific journal of cancer prevention : APJCP·2025
Same journal

Ligno-Suberized Fruit Periderm as a Mechanically Reinforced Biomacromolecular Composite.

Biomacromolecules·2026
Same journal

Effect of Hydrophilic Brush Length and Hydrophobic Chain on Biodistribution of Polymethacrylate-Based Statistical Copolymers.

Biomacromolecules·2026
Same journal

Multicomponent Micelles with Boosted Stability of Iminoboronates.

Biomacromolecules·2026
Same journal

Stiffening and Toughening Protein Hydrogels by Tuning Electrostatic Interactions.

Biomacromolecules·2026
Same journal

<i>In Situ</i> Bulk and Interfacial Interlocking-Induced Highly Dynamically Entangled Hydrogel of Myocardium-Matching Mechanics, Electrophysiological Functions, and Robust Tissue Adhesion for Cardiac Repair.

Biomacromolecules·2026
Same journal

Eutectogel Electrodes with Self-powered Capability for Flexible Electrophysiological Sensor.

Biomacromolecules·2026
See all related articles

Related Experiment Video

Updated: Jan 14, 2026

Author Spotlight: Optimization of Ultrashort Peptide Matrices for Colorectal Cancer Organoids
10:23

Author Spotlight: Optimization of Ultrashort Peptide Matrices for Colorectal Cancer Organoids

Published on: May 3, 2024

1.4K

Enzyme-Instructed Self-Assembly of Endoplasmic Reticulum-Targeting Peptides for Selective Modulation of Cancer Cell

Jihun H Roh1, Priyanka Upadhyay1, Chae-Young Lee2

  • 1Department of Chemistry, University of Ulsan, Ulsan 44776, Republic of Korea.

Biomacromolecules
|October 17, 2025
PubMed
Summary
This summary is machine-generated.

Enzyme-instructed self-assembly (EISA) targeting the endoplasmic reticulum (ER) selectively kills cancer cells. This ER-targeting peptide strategy enhances cancer cell death and overcomes concentration challenges of traditional intracellular EISA.

More Related Videos

Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro
10:01

Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro

Published on: April 8, 2020

6.3K
A Tripeptide-Stabilized Nanoemulsion of Oleic Acid
10:42

A Tripeptide-Stabilized Nanoemulsion of Oleic Acid

Published on: February 27, 2019

9.8K

Related Experiment Videos

Last Updated: Jan 14, 2026

Author Spotlight: Optimization of Ultrashort Peptide Matrices for Colorectal Cancer Organoids
10:23

Author Spotlight: Optimization of Ultrashort Peptide Matrices for Colorectal Cancer Organoids

Published on: May 3, 2024

1.4K
Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro
10:01

Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro

Published on: April 8, 2020

6.3K
A Tripeptide-Stabilized Nanoemulsion of Oleic Acid
10:42

A Tripeptide-Stabilized Nanoemulsion of Oleic Acid

Published on: February 27, 2019

9.8K

Area of Science:

  • Biomedical Engineering
  • Cancer Biology
  • Drug Delivery

Background:

  • Enzyme-instructed self-assembly (EISA) is a promising strategy for cancer therapy.
  • Targeting specific organelles like the endoplasmic reticulum (ER) can enhance therapeutic efficacy.
  • Intracellular EISA faces challenges related to concentration dependency and specificity.

Purpose of the Study:

  • To develop a novel peptide for targeted endoplasmic reticulum (ER) delivery using EISA.
  • To investigate the selective accumulation and cancer cell-killing effects of ER-targeting EISA.
  • To compare the efficacy of ER-targeting EISA with conventional intracellular EISA.

Main Methods:

  • Conjugation of a p-toluenesulfonamide moiety (ER-targeting) to an alkaline phosphatase (ALP)-responsive peptide.
  • Utilizing ALP's presence in cancer cells to trigger peptide self-assembly and ER accumulation.
  • Evaluating selective cancer cell death induction (apoptosis and necroptosis) via ER stress and dysfunction.

Main Results:

  • The developed peptide selectively accumulated on the ER in cancer cells with high ALP expression.
  • ER-targeting EISA induced significant ER stress and dysfunction, leading to cancer cell death.
  • ER-targeting EISA demonstrated a >2-fold lower IC50 compared to non-targeted intracellular EISA, overcoming concentration limitations.

Conclusions:

  • ER-targeting EISA represents a potent strategy for selective cancer cell elimination.
  • This approach enhances therapeutic efficiency by concentrating the EISA at the ER organelle.
  • The study highlights the potential of organelle-specific EISA for improved cancer therapy.