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Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...

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Visualizing Angiogenesis by Multiphoton Microscopy In Vivo in Genetically Modified 3D-PLGA/nHAp Scaffold for Calvarial Critical Bone Defect Repair
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Mir-27 Promotes Periodontal Regeneration via Osteogenesis/Angiogenesis.

Y Ding1,2, J Luan1,3, H S Malmstrom4

  • 1University of Rochester School of Medicine and Dentistry, Eastman Institute for Oral Health, Department of Oral and Craniofacial Sciences, Rochester, NY, USA.

Journal of Dental Research
|October 17, 2025
PubMed
Summary
This summary is machine-generated.

MicroRNA-27 (miR-27) delivered via lipid nanoparticles (LNPs) promotes periodontal tissue regeneration by enhancing both bone formation (osteogenesis) and blood vessel growth (angiogenesis). This approach targets Secreted Frizzled-Related Protein 1 (SFRP1), offering a novel treatment for periodontal disease.

Keywords:
Wnt signaling pathwaymicroRNAnanomaterialsperiodontal diseasetissue engineeringtissue regeneration

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Area of Science:

  • Biomedical Engineering
  • Regenerative Medicine
  • Molecular Biology

Background:

  • Successful bone regeneration requires coordinated osteogenesis and angiogenesis.
  • The Wnt signaling pathway is crucial for both bone and blood vessel development.
  • Periodontal disease involves inflammation that can impair regenerative processes.

Purpose of the Study:

  • To evaluate the potential of microRNA-27 (miR-27) delivered by lipid nanoparticles (LNPs) for periodontal tissue regeneration.
  • To investigate the mechanism of miR-27 in modulating osteogenesis and angiogenesis via Secreted Frizzled-Related Protein 1 (SFRP1).
  • To assess the efficacy of miR-27-LNPs in a periodontitis model.

Main Methods:

  • Delivery of miR-27 mimic using lipid nanoparticles (LNPs).
  • Assessment of osteogenic markers (ALP, RUNX2, COL1) and angiogenic factors (CD31, CD34, VEGF) expression.
  • In vitro assays for alkaline phosphatase activity, mineral deposition, spheroid sprouting, and tube formation.
  • In vivo studies evaluating extracellular matrix remodeling, neovascularization, and periodontal tissue/bone regeneration.

Main Results:

  • miR-27 treatment upregulated key osteogenic and angiogenic markers in a periodontitis model.
  • Enhanced alkaline phosphatase activity, mineral deposition, and improved in vitro angiogenesis observed.
  • In vivo implantation showed accelerated matrix remodeling, a 6-fold increase in neovascularization, and significant periodontal regeneration.
  • miR-27 promoted regeneration by suppressing SFRP1, thereby activating the Wnt pathway.

Conclusions:

  • miR-27 mimic, delivered via LNPs, effectively promotes periodontal tissue regeneration by enhancing coupled osteogenesis and angiogenesis.
  • The miR-27-SFRP1-Wnt signaling axis is a key mechanism underlying this regenerative effect.
  • The miR-27-LNPs/scaffold combination represents a promising therapeutic strategy for periodontal disease treatment.