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Bifunctional Fluorescence Aptasensor Based on Target-Induced Strand Displacement Enables Simultaneous Detection of
M A Hagir Omer1,2, Meng Li1, Xiaolin Yang1
1Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, P.R. China.
Analytical Chemistry
|October 17, 2025
Summary
A new fluorescence aptasensor enables simultaneous detection of dopamine (DA) and serotonin (5-HT) in body fluids. This simple, sensitive platform offers a promising tool for early neurological disease diagnosis at the point-of-care.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Neuroscience
Background:
- Simultaneous detection of dopamine (DA) and serotonin (5-HT) is crucial for early neurological disease diagnosis.
- Current methods require complex laboratory setups, limiting accessibility.
Purpose of the Study:
- To develop a bifunctional fluorescence aptasensor for simultaneous DA and 5-HT detection.
- To enable point-of-care testing in various body fluids.
Main Methods:
- Utilized target-induced strand displacement with dual-labeled aptamers (Cy5/FAM) and a quencher.
- Developed a solution-based assay and a paper-based aptasensor on glass fiber paper (GFP).
- Validated detection in artificial cerebrospinal fluid, urine, and tears against HPLC.
Main Results:
- Achieved high sensitivity with limits of detection of 0.3 nM for DA and 0.5 nM for 5-HT.
- Demonstrated good recovery rates in artificial cerebrospinal fluid, urine, and tears.
- Showcased results consistent with high-performance liquid chromatography (HPLC) analysis.
Conclusions:
- The developed bifunctional fluorescence aptasensor provides a simple and sensitive method for neurotransmitter detection.
- The paper-based format offers a promising point-of-care platform for diagnosing neurological diseases.
- This technology facilitates the analysis of multiple neurotransmitters in diverse biological samples.
