Related Experiment Video

Updated: Jan 14, 2026

Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor
07:16

Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor

Published on: September 13, 2018

20.6K

IL-19 suppresses Hippo signaling via modulating YAP1 phosphorylation in osteoarthritis

Jiezhong Deng1, Jun Gu2,3, Xiaoshan Gong2

  • 1Department of Orthopedics, Southwest Hospital, Army Medical University, Chongqing, China.

Frontiers in Immunology
|October 17, 2025
PubMed
Abstract

Insights

Interleukin-19 (IL-19) delays osteoarthritis progression by reducing inflammation and chondrocyte apoptosis. This study found IL-19 inhibits the Hippo signaling pathway, offering a potential therapeutic target for osteoarthritis.

Area of Science:

  • Immunology
  • Rheumatology
  • Cell Biology

Background:

  • Osteoarthritis (OA) is a prevalent degenerative joint disease.
  • Chondrocyte dysfunction, apoptosis, and cartilage fibrosis characterize OA.
  • Interleukin-19 (IL-19) has known anti-inflammatory and tissue-repair roles, but its effect on OA is unexplored.

Purpose of the Study:

  • To investigate the role and therapeutic potential of IL-19 in osteoarthritis.
  • To determine if IL-19 can mitigate inflammation and apoptosis in osteoarthritis models.

Main Methods:

  • Established Interleukin 1β (IL-1β)-induced inflammation and ACLT-induced osteoarthritis mouse models.
  • Quantified IL-19 expression in OA joints and identified its cellular source (synovial M2 macrophages).
  • Assessed the impact of IL-19 on the Hippo signaling pathway and YAP1 phosphorylation.

Main Results:

  • IL-19 expression was elevated in the joints of OA mice, originating from M2 macrophages.
  • IL-19 demonstrated anti-inflammatory and anti-apoptotic effects on IL-1β-induced chondrocytes.
  • IL-19 mitigated osteoarthritis progression by inhibiting the Hippo signaling pathway and YAP1 phosphorylation.

Conclusions:

  • IL-19 plays a protective role in osteoarthritis by suppressing inflammation and chondrocyte apoptosis.
  • Inhibition of the Hippo signaling pathway by IL-19 is a key mechanism in its OA-protective effects.
  • IL-19 represents a potential therapeutic agent for delaying osteoarthritis progression.
Keywords:
Hippo-yapIL-19M2 macrophagesOAapoptosisinflammation

More Related Videos

Culturing and Manipulation of O9-1 Neural Crest Cells08:32

Culturing and Manipulation of O9-1 Neural Crest Cells

Published on: October 9, 2018

10.9K
Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter11:32

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

Published on: March 27, 2020

7.4K

Related Concept Videos

Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
9.8K
The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
12.0K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
5.3K
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
10.5K
T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
2.1K
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
7.2K