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  1. Home
  3. Lab-scale Production Of Postbiotic Proteins From Bifidobacterium Adolescentis With Antiviral And Epithelial-protective Properties.
  1. Home
  3. Lab-scale Production Of Postbiotic Proteins From Bifidobacterium Adolescentis With Antiviral And Epithelial-protective Properties.

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Lab-scale production of postbiotic proteins from Bifidobacterium adolescentis with antiviral and

María Hernández1, Balkys Quevedo2, María Cabrera1

  • 1Grupo de Enfermedades Infecciosas, Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Colombia.

Frontiers in Microbiology
|October 17, 2025

View abstract on PubMed

Summary
This summary is machine-generated.

This study shows that protein-rich postbiotics from Bifidobacterium adolescentis are safe and can reduce rotavirus infection. These compounds may benefit intestinal health and manage viral infections.

Keywords:
Bifidobacterium adolescentisantiviral activitybioreactorcytoskeletal integrityfunctional ingredientsgut epitheliumpostbioticsrotavirus

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Area of Science:

  • Microbiology
  • Biotechnology
  • Gastroenterology

Background:

  • Postbiotics, derived from probiotic bacteria, are emerging as valuable food-derived agents for health applications.
  • Bifidobacterium adolescentis is a key probiotic with potential for producing beneficial postbiotics.
  • Developing scalable methods for postbiotic production is crucial for functional ingredient development.

Purpose of the Study:

  • To investigate the production and biological activity of protein-rich postbiotics from Bifidobacterium adolescentis.
  • To assess the safety and functional efficacy of these postbiotics in managing intestinal health and viral infections.

Main Methods:

  • Cultivation of Bifidobacterium adolescentis in a 3-liter bioreactor with optimized parameters.
  • Cytotoxicity assays using MA104 (renal) and C2BBe1 (intestinal) epithelial cell lines.
  • Evaluation of anti-rotavirus activity and epithelial integrity preservation in infected intestinal cells.

    • Main Results:

    • The postbiotic preparation demonstrated safety, showing no cytotoxicity across a range of concentrations in epithelial cell lines.
    • Significant reduction in rotavirus infectivity was observed in the presence of the postbiotic.
    • The postbiotic maintained cytoskeletal architecture in infected intestinal cells, indicating a protective effect.

    Conclusions:

    • Bifidobacterium adolescentis-derived postbiotics are safe and possess biological activity relevant to intestinal health.
    • These postbiotics show potential for managing viral infections, particularly rotavirus.
    • The findings support the development of B. adolescentis postbiotics as functional ingredients for health applications.