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Adaptation of swinepox virus to an established cell line
Applied Microbiology
|January 1, 1972
Abstract:
Swinepox virus was successfully adapted to the PK-15 cell line, but not the MDBK cell line. Virus titers obtained in the PK-15 host cell system were comparable to diploid swine kidney cell cultures.
Insights
Swinepox virus adapted to PK-15 cells, not MDBK cells. Titers in PK-15 cells matched those in swine kidney cell cultures, indicating successful viral propagation.
Area of Science:
- Veterinary Virology
- Cell Culture Technology
Background:
- Swinepox virus (SPV) is an important pathogen affecting swine health.
- Effective cell culture systems are crucial for SPV research and vaccine development.
Purpose of the Study:
- To evaluate the suitability of PK-15 and MDBK cell lines for Swinepox virus propagation.
- To compare virus titers achieved in adapted cell lines with traditional methods.
Main Methods:
- Swinepox virus was inoculated into PK-15 and MDBK cell cultures.
- Virus adaptation and propagation were monitored.
- Virus titers were determined in successfully adapted cell lines.
Main Results:
- Swinepox virus successfully adapted and replicated in the PK-15 cell line.
- Swinepox virus did not adapt to the MDBK cell line.
- Virus titers in the PK-15 cell system were comparable to those obtained in diploid swine kidney cell cultures.
Conclusions:
- The PK-15 cell line is a suitable host system for Swinepox virus propagation.
- The MDBK cell line is not suitable for Swinepox virus adaptation.
- PK-15 cells offer a viable alternative for Swinepox virus research and potentially for vaccine production.