Semi-micro XC cell assay technique for murine leukemia virus

Area of Science:

• Virology
• Cell Biology
• Biotechnology

Background:

• The XC cell assay is a standard method for in vitro titration of murine leukemia viruses.
• Existing protocols can be resource-intensive and time-consuming.

Purpose of the Study:

• To adapt the XC cell assay for a semi-micro procedure.
• To enhance the efficiency and practicality of murine leukemia virus titration.

Main Methods:

• Modification of the standard XC cell assay protocol.
• Implementation of a semi-micro format for cell culture and analysis.

Main Results:

• The modified XC cell assay successfully performs in vitro titration of murine leukemia viruses.
• The semi-micro procedure offers a more streamlined approach to viral quantification.

Conclusions:

• The adapted XC cell assay provides a viable and efficient semi-micro alternative for murine leukemia virus titration.
• This modification facilitates broader application and potentially reduces experimental costs.