Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Targeting SYK to alleviate MDSC-driven immunosuppression and augment anti-PD1 efficacy.

Science China. Life sciences·2026
Same author

Eu<sup>3+</sup>-mediated ratiometric fluorescence visualization sensing for traceability and content prediction of plant-derived products: an example of Chrysanthemi Flos.

Talanta·2026
Same author

BIRC3 (Encoding Cellular Inhibitor of Apoptosis Protein 2) Variants Result in Dysregulated Receptor-Interacting Protein Kinase 1 Signaling Leading to Increased Epithelial Cell Death and Are Associated With Monogenic Crohn's Disease.

Gastroenterology·2026
Same author

Author Correction: Increasing the efficiency and targeting range of cytidine base editors through fusion of a single-stranded DNA-binding protein domain.

Nature cell biology·2026
Same author

Bufalin inhibits proliferation of non-small cell lung cancer by promoting metabolic reprogramming through inhibition of pyruvate kinase M2.

Biochemical pharmacology·2026
Same author

Constructing Internal Electric Field at Perovskite Heterojunction Interfaces and Electron Transfer-Enhanced Catalytic Soot Combustion.

Langmuir : the ACS journal of surfaces and colloids·2026

Related Experiment Video

Updated: Jan 14, 2026

Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein
05:48

Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein

Published on: March 16, 2022

3.0K

Construction of pathogenic Sec16a mutation mouse model using CRISPR/Cas9.

Yaqiang Hu1, Zhiyang Zeng2, Xinyu Ming1

  • 1Shanghai Frontiers Science Center of Genome Editing and Cell Therapy, Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai, China.

Animal Models and Experimental Medicine
|October 17, 2025
PubMed
Summary
This summary is machine-generated.

A new mouse model with a SEC16A mutation shows impaired learning and memory, along with clasping behavior. This model aids in understanding brain dysfunction caused by SEC16A gene mutations.

Keywords:
Sec16aCRISPR/Cas9endoplasmic reticulum stressmouse modelneurodegenerative diseases

More Related Videos

Generation of Brown Fat-Specific Knockout Mice Using a Combined Cre-LoxP, CRISPR-Cas9, and Adeno-Associated Virus Single-Guide RNA System
03:58

Generation of Brown Fat-Specific Knockout Mice Using a Combined Cre-LoxP, CRISPR-Cas9, and Adeno-Associated Virus Single-Guide RNA System

Published on: March 24, 2023

3.1K
Adeno-Associated Virus-Mediated Delivery of CRISPR for Cardiac Gene Editing in Mice
09:00

Adeno-Associated Virus-Mediated Delivery of CRISPR for Cardiac Gene Editing in Mice

Published on: August 2, 2018

8.6K

Related Experiment Videos

Last Updated: Jan 14, 2026

Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein
05:48

Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein

Published on: March 16, 2022

3.0K
Generation of Brown Fat-Specific Knockout Mice Using a Combined Cre-LoxP, CRISPR-Cas9, and Adeno-Associated Virus Single-Guide RNA System
03:58

Generation of Brown Fat-Specific Knockout Mice Using a Combined Cre-LoxP, CRISPR-Cas9, and Adeno-Associated Virus Single-Guide RNA System

Published on: March 24, 2023

3.1K
Adeno-Associated Virus-Mediated Delivery of CRISPR for Cardiac Gene Editing in Mice
09:00

Adeno-Associated Virus-Mediated Delivery of CRISPR for Cardiac Gene Editing in Mice

Published on: August 2, 2018

8.6K

Area of Science:

  • Molecular Biology
  • Neuroscience
  • Genetics

Background:

  • SEC16A protein is crucial for protein transport from the endoplasmic reticulum to the Golgi apparatus.
  • A specific mutation (c.4606C>G) in SEC16A was identified within a conserved region.
  • SEC16A is integral to the COPII complex assembly.

Purpose of the Study:

  • To develop a mouse model for studying the pathogenic mechanisms of SEC16A mutations.
  • To investigate the role of SEC16A in neurological function and brain diseases.

Main Methods:

  • Developed the Sec16aL1551V/L1551V mouse model using CRISPR/Cas9 technology.
  • Aligned human and mouse protein sequences to guide model creation.
  • Utilized behavioral experiments like novel object recognition and cued fear conditioning.

Main Results:

  • Sec16aL1551V/L1551V mice exhibited impaired learning and memory.
  • Mice displayed a characteristic limb clasping behavior during tail suspension.
  • This phenotype suggests neurological impairment and potential neurodegeneration.

Conclusions:

  • The Sec16aL1551V/L1551V mouse model is a valuable tool for studying SEC16A-related brain dysfunction.
  • This model facilitates research into the pathogenic mechanisms of SEC16A gene mutations.
  • Provides insights into neurodegenerative disease models.