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A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

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One-Step, Signal-On, and Modification-Free Electrochemical Sensing Platform Based on Aptamer Switch Hydrogel.

Yin Bao^1,2, Guoqi Ding^1,2, Yulin Li^1,2

¹Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.

Analytical Chemistry

|October 17, 2025

Summary

This study introduces a novel aptamer-based DNA hydrogel electrochemical sensor (Apt-HE-Sensor) for simple, signal-on detection. This innovative platform enhances reproducibility and practicality for environmental and health monitoring.

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Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Published on: March 21, 2018

Area of Science:

Electrochemistry
Biomolecular Engineering
Materials Science

Background:

Electrochemical sensors often face challenges with complex fabrication, operation, and off-signaling modes, limiting their reproducibility and practical use.
Existing methods require electrode modification, liquid transfer, and washing steps, adding complexity to sensor development and application.

Purpose of the Study:

To develop a novel aptamer-based, target-responsive DNA hydrogel electrochemical sensing platform (Apt-HE-Sensor).
To enable one-step, signal-on target detection, simplifying sensor operation and improving practicality.
To demonstrate a universal platform for detecting various small molecules and biomolecules.

Main Methods:

Integration of an aptamer-embedded DNA hydrogel as a barrier layer on a bare electrode.
Utilizing target recognition at the solid-liquid interface to trigger hydrogel decomposition.
Monitoring the accelerated diffusion of electroactive probes to the electrode surface upon target binding.

Main Results:

The Apt-HE-Sensor platform demonstrated a wide linear range (1-1500 μmol/L) and a low detection limit (0.58 μmol/L) for tetracycline detection.
The sensor maintained excellent detection performance in real water samples.
The platform showed significant target discrimination by aptamer substitution, indicating broad applicability.

Conclusions:

The Apt-HE-Sensor offers a straightforward, reproducible, and practical solution for electrochemical sensing.
This method facilitates on-site, real-time monitoring of analytes in environmental and health applications.
The DNA hydrogel platform presents a versatile approach for developing next-generation biosensors.