Naringenin-Loaded Core-Shell Mg-MOF Nanocomposite Integrated Injectable Hydrogel for Bone Regeneration:...

Area of Science:

• Biomaterials Science
• Regenerative Medicine
• Nanotechnology

Background:

• Composite biomaterials offer synergistic advantages for tissue regeneration.
• Developing advanced materials for bone defect repair remains a critical challenge.
• Current strategies often lack multi-faceted therapeutic delivery for optimal healing.

Purpose of the Study:

• To develop novel core-shell nanocomposites (N-HMMs) for enhanced bone regeneration.
• To investigate the synergistic effects of naringin, Mg2+, and gallic acid release.
• To evaluate the efficacy of the N-HMMs@Nar@GP composite in a bone defect model.

Main Methods:

• Fabrication of hollow mesoporous silica (HMSNs) core and magnesium-gallic acid metal-organic framework (Mg-MOF) shell nanocomposites (N-HMMs).
• Loading of naringin (Nar) into N-HMMs to create N-HMMs@Nar.
• Integration of N-HMMs@Nar into methacrylate gelatin/polyethylene glycol diacrylate (GelMA/PEGDA, GP) hydrogels to form N-HMMs@Nar@GP.
• In vitro assessment of biocompatibility, osteogenic differentiation, and angiogenesis.
• In vivo evaluation in a rat tibial defect model using micro-CT and histopathology.

Main Results:

• N-HMMs@Nar@GP demonstrated sustained release of naringin, Mg2+, and gallic acid.
• In vitro studies confirmed excellent biocompatibility, osteoinductive, and angiogenic potential.
• In vivo studies showed enhanced osteogenesis and angiogenesis in tibial defects, confirming therapeutic efficacy.

Conclusions:

• The developed N-HMMs@Nar@GP composite biomaterial effectively promotes bone regeneration.
• Synergistic therapeutic effects of released compounds contribute to anti-inflammation, osteogenesis, and angiogenesis.
• This advanced hydrogel system shows significant promise for clinical applications in bone defect repair.