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Hesperidin Attenuates Acrylamide-Induced Ovarian Toxicity in Rats: Based on Network Pharmacology, Molecular Docking,

Divya Gupta1, Sadhana Shrivastava1, Chakresh Kumar Jain2

  • 1Reproductive Biology and Toxicology Lab, School of Studies in Zoology, Jiwaji University, Gwalior, India.

Environmental Toxicology
|October 17, 2025
PubMed

Insights

Hesperidin (HES) protects against acrylamide (AA)-induced ovarian toxicity in rats. This plant-derived compound demonstrates significant antioxidant properties, offering a potential therapeutic strategy against food-borne toxins.

Area of Science:

  • Toxicology
  • Pharmacology
  • Biochemistry

Background:

  • Acrylamide (AA) exposure from food is widespread and linked to reproductive toxicity, including reduced ovarian weight and follicle count.
  • Flavonoids, like hesperidin (HES), are plant-derived bioactive compounds with potential medicinal applications.

Purpose of the Study:

  • To investigate the protective effects of hesperidin (HES) against acrylamide (AA)-induced ovarian damage in a rat model.
  • To identify potential target genes and pathways involved in HES therapy and AA toxicity using bioinformatics analysis.
  • To assess the molecular interactions between AA, HES, and core target genes via molecular docking.

Main Methods:

  • Rats were orally administered acrylamide (40 mg/kg) for 10 days, followed by varying doses of hesperidin for three days.
  • Evaluation included reproductive hormone levels, oxidative stress markers, membrane-bound enzymes, glycogen content, and histological examination.
  • Bioinformatic analyses, including functional enrichment and pathway analysis, were performed on identified target genes. Molecular docking was utilized to study gene-ligand interactions.

Main Results:

  • Hesperidin (HES) exhibited significant protective effects against acrylamide (AA)-induced ovarian damage in vivo.
  • HES demonstrated strong antioxidant properties, mitigating oxidative stress markers and preserving ovarian function.
  • Molecular docking revealed a high binding affinity between HES and core target proteins, suggesting a direct mechanism of action.

Conclusions:

  • Hesperidin possesses significant protective capabilities against acrylamide-induced reproductive toxicity in rats.
  • The antioxidant activity of HES is a key factor in its protective mechanism against AA-induced ovarian damage.
  • HES represents a promising therapeutic agent for mitigating the adverse effects of dietary acrylamide exposure.
Keywords:
PPI network analysisacrylamidehesperidinmolecular dockingovarian toxicityoxidative stress

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