Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

20.0K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
20.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Distinct prognostic value of aspartate-to-alanine aminotransferase ratio (AAR) in traumatic brain injury versus hemorrhagic stroke: a cohort study of 1,069 patients.

Frontiers in neurology·2026
Same author

Preliminary Cold Tolerance Evaluation of Seven <i>Ilex</i> Species Based on Physiological Responses of Detached Leaves to Acute Low-Temperature Stress.

Plants (Basel, Switzerland)·2026
Same author

Modulating p53 condensation rewires transcriptional programs to enhance tumor suppression.

Molecular therapy : the journal of the American Society of Gene Therapy·2026
Same author

Focus-guided feature fusion network for lightweight image super-resolution.

Neural networks : the official journal of the International Neural Network Society·2026
Same author

Analysis of the Chief Complaints at Consultation in 278 Patients With Submucous Cleft Palate.

The Journal of craniofacial surgery·2026
Same author

Post-transcriptional effects of <i>leptin</i> 3'UTR polymorphisms on Milk traits in dairy cattle.

Food chemistry. Molecular sciences·2026
Same journal

Immunofluorescence study and morphometric analysis of collagen-IV in the exchange tissue of the quail (Coturnix coturnix) lung by confocal laser scanning microscopy.

Micron (Oxford, England : 1993)·2026
Same journal

Micromorphological study of leaf surfaces structures in selected Crataegus L. (Rosaceae) species using light, scanning electron, and confocal laser microscopy.

Micron (Oxford, England : 1993)·2026
Same journal

3D reconstruction of the nymphal feeding apparatus of Philaenus spumarius.

Micron (Oxford, England : 1993)·2026
Same journal

The influence of physicians and surgeons on Leeuwenhoek's observations of crystal formation.

Micron (Oxford, England : 1993)·2026
Same journal

Distribution of telocytes in the choroid (eye) of a teleost: An ultrastructural observation.

Micron (Oxford, England : 1993)·2026
Same journal

SEM-EDAX: A tool for microanalytical elemental mapping in butterfly wing scales.

Micron (Oxford, England : 1993)·2026
See all related articles

Related Experiment Video

Updated: Jan 14, 2026

Author Spotlight: A Machine-Vision Approach to Transmission Electron Microscopy Workflows, Results Analysis and Data Management
10:23

Author Spotlight: A Machine-Vision Approach to Transmission Electron Microscopy Workflows, Results Analysis and Data Management

Published on: June 23, 2023

3.5K

Focus measure through vectorization analysis for microscopic shape from focus.

Yuezong Wang1, Haoran Jia2

  • 1College of Mechanical and Energy Engineering, Beijing University of Technology, Beijing 100124, China.

Micron (Oxford, England : 1993)
|October 17, 2025
PubMed
Summary
This summary is machine-generated.

This study introduces a new focus measure for Shape from Focus (SFF) to reconstruct 3D micro-scale object shapes. The gradient vector approach improves accuracy for complex surfaces and enables faster large-scale reconstructions.

Keywords:
Focus measureGradient vectorLarge-scale sceneShape from focus

More Related Videos

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

Published on: February 8, 2014

12.7K
A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules
10:45

A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules

Published on: June 20, 2020

10.8K

Related Experiment Videos

Last Updated: Jan 14, 2026

Author Spotlight: A Machine-Vision Approach to Transmission Electron Microscopy Workflows, Results Analysis and Data Management
10:23

Author Spotlight: A Machine-Vision Approach to Transmission Electron Microscopy Workflows, Results Analysis and Data Management

Published on: June 23, 2023

3.5K
Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

Published on: February 8, 2014

12.7K
A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules
10:45

A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules

Published on: June 20, 2020

10.8K

Area of Science:

  • Microscopy
  • Computational Imaging
  • 3D Reconstruction

Background:

  • Shape from Focus (SFF) reconstructs 3D shapes from multi-focus images.
  • Traditional SFF struggles with micro-scale objects, complex surfaces, and limited fields of view.
  • High magnification imaging presents challenges for accurate 3D surface reconstruction.

Purpose of the Study:

  • To develop an advanced focus measure for precise 3D Shape from Focus (SFF).
  • To overcome limitations of traditional methods in reconstructing micro-scale objects with complex surfaces.
  • To enhance efficiency for large-scale 3D surface reconstruction.

Main Methods:

  • Transformed grayscale images to gradient vector (GV) space.
  • Applied divergence and curl operations in GV space to identify optimal focus regions.
  • Developed strategies for temporal efficiency in large-scale scene reconstruction and multi-field image acquisition.

Main Results:

  • The novel focus measure effectively handles complex surface structures and height mutations.
  • Improved precision and robustness in 3D shape reconstruction of micro-scale objects.
  • Enabled rapid surface shape computation for micro-scale objects within a short timeframe.

Conclusions:

  • The proposed gradient vector-based focus measure significantly enhances Shape from Focus (SFF) capabilities.
  • This method offers a robust solution for 3D reconstruction of challenging micro-scale surfaces.
  • The strategies improve efficiency for extensive area reconstruction in micro-scale imaging.