Analysis of the tumor immune microenvironment of irradiated brain tumors by flow cytometry

Strategies to enhance the recruitment of immune cells in brain tumors to restore its sensitivity to immunotherapy is an area of active investigation. In that context, flow cytometry analysis that assesses the brain immune contexture before and after treatment is critical for developing approaches that foster an inflammatory phenotype of brain tumors. In this chapter, we present a flow cytometry protocol that can be utilized to determine the immune landscape of brain tumors before and after treatment in preclinical models.