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Related Experiment Video

Updated: Jan 14, 2026

Four-color Fluorescence Immunohistochemistry of T-cell Subpopulations in Archival Formalin-fixed, Paraffin-embedded Human Oropharyngeal Squamous Cell Carcinoma Samples

Four-color Fluorescence Immunohistochemistry of T-cell Subpopulations in Archival Formalin-fixed, Paraffin-embedded Human Oropharyngeal Squamous Cell Carcinoma Samples

Published on: July 29, 2017

A Practical Method for Detecting Prognostic CD57+ T Cells in Head and Neck Cancer.

Brianna Brammer^1,2, Brendan L C Kinney^1,2, Vikash Kansal^1,2

¹Department of Otolaryngology-Head and Neck Surgery, Emory University, Atlanta, Georgia, USA.

|October 17, 2025

Summary

Predicting head and neck squamous cell carcinoma (HNSCC) recurrence is crucial. Elevated highly differentiated, CD57+ effector memory T (HD TEMRA) cells can predict recurrence, aiding personalized treatment planning.

Keywords:

CD57 TEMRA cells biomarker flow cytometry head and neck cancer recurrence

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Last Updated: Jan 14, 2026

Four-color Fluorescence Immunohistochemistry of T-cell Subpopulations in Archival Formalin-fixed, Paraffin-embedded Human Oropharyngeal Squamous Cell Carcinoma Samples

Four-color Fluorescence Immunohistochemistry of T-cell Subpopulations in Archival Formalin-fixed, Paraffin-embedded Human Oropharyngeal Squamous Cell Carcinoma Samples

Published on: July 29, 2017

Isolation and Characterization of a Head and Neck Squamous Cell Carcinoma Subpopulation Having Stem Cell Characteristics

Isolation and Characterization of a Head and Neck Squamous Cell Carcinoma Subpopulation Having Stem Cell Characteristics

Published on: May 11, 2016

Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry

Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry

Published on: March 5, 2019

Area of Science:

Immunology
Oncology
Flow Cytometry

Background:

Head and neck squamous cell carcinoma (HNSCC) recurrence impacts survival in nearly half of patients.
Current methods lack robust prediction of HNSCC recurrence.
Elevated circulating highly differentiated, CD57+ effector memory T (HD TEMRA) cells correlate with early recurrence and poor outcomes in HNSCC.

Purpose of the Study:

To present refined flow cytometry panels for predicting HNSCC recurrence.
To simplify the identification of HD TEMRA cells as a prognostic biomarker.

Main Methods:

Utilized refined, less complex flow cytometry panels.
Identified HD TEMRA cells as live CD8+ CD28- CD57+ cells.
Analyzed a cohort of surgically treated HNSCC patients.

Main Results:

Refined flow cytometry panels effectively predicted HNSCC recurrence.
The proportion of HD TEMRA cells (CD8+ CD57+) predicted locoregional recurrence in oral squamous cell carcinoma patients.
Simplified panels retained prognostic value.

Conclusions:

Refined panels enhance the clinical utility of HD TEMRA cell proportion as a biomarker.
Simplified identification of HD TEMRA cells improves technical and analytic ease.
Implementing HD TEMRA cell proportion can personalize HNSCC treatment strategies.