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Multifunctional Primer for Dentin Bonding via Biomimetic Mineralization
S H Yang1, Z L Tian1, H M Wang2
1Jilin Provincial Key Laboratory of Oral Biomedical Engineering, Hospital of Stomatology, Jilin University, Changchun, China.
Journal of Dental Research
|October 18, 2025
Summary
Aldehyde-grafted polyaspartate (PACA) enhances dental restoration durability by regulating biomimetic mineralization and collagen cross-linking. This innovative primer improves bond strength and longevity, offering a solution for dental adhesives.
Area of Science:
- Biomaterials Science
- Dental Materials
- Nanotechnology
Background:
- Dentin bonding durability is a major challenge in dental restorations.
- Current adhesive systems often degrade over time, compromising restoration longevity.
- Biomimetic mineralization offers a potential strategy to enhance dentin bonding integrity.
Purpose of the Study:
- To introduce aldehyde-grafted polyaspartate (PACA) as a novel agent for spatiotemporal control of biomimetic mineralization.
- To investigate PACA's ability to cross-link collagen and induce intrafibrillar mineralization.
- To evaluate PACA's efficacy as a single-component primer for improving dentin bonding durability.
Main Methods:
- Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) to confirm collagen cross-linking.
- Dynamic light scattering (DLS), zeta potential, and transmission electron microscopy (TEM) to study intrafibrillar mineralization.
- Scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), and atomic force microscopy (AFM) for remineralization evaluation.
- Micro-tensile bond strength testing, nanoleakage analysis, and in situ zymography to assess bonding durability.
Main Results:
- PACA effectively cross-links collagen fibrils and induces intrafibrillar mineralization, creating an amorphous calcium phosphate-rich microenvironment.
- Mineralization within PACA-treated collagen forms an organized crystalline structure, encapsulating the polymer and collagen.
- As a single-component primer, PACA simultaneously achieved collagen cross-linking, MMP inhibition, and enhanced adhesive penetration.
- PACA significantly improved micro-tensile bond strength and demonstrated favorable durability after aging.
Conclusions:
- PACA facilitates controlled biomimetic mineralization and collagen cross-linking, enhancing dentin bonding.
- The multifunctional nature of PACA provides a promising strategy to overcome the durability limitations of conventional dental adhesive systems.
- This approach offers a novel solution for long-lasting and robust dental restorations.
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