Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

2.4K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.4K
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

8.3K
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
8.3K
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

10.4K
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
10.4K
Notch Signaling Pathway03:14

Notch Signaling Pathway

6.4K
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
6.4K
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

3.3K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
3.3K
Whole Body Regeneration01:33

Whole Body Regeneration

4.0K
Regeneration is the process of restoring injured or lost tissues, organs, or body parts. While simpler organisms generally show greater ability to regenerate their whole body, few complex animals show similarly exceptional regeneration. For example, planarian flatworms have a unique regenerative potential making them a popular study organism among biologists to understand the mechanisms of whole body regeneration. Other organisms, such as hydra, also show extreme regeneration potential;...
4.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Influence of organic loading rate on bioflocculant production derived from waste glycerin pitch and mixed microbial culture in single-step system.

Bioresource technology·2026
Same author

Progressive tooth pattern changes in Cilk1-deficient mice depending on Hedgehog signaling.

International journal of oral science·2025
Same author

Notum as a Crucial Regulator of Matrix Integrity in Dentinogenesis.

Journal of cellular physiology·2025
Same author

Dermoscopy for lipidized dermatofibroma: A useful diagnostic tool.

Annales de dermatologie et de venereologie·2024
Same author

Low household income increases the risk of tuberculosis recurrence: a retrospective nationwide cohort study in South Korea.

Public health·2023
Same author

Impaired breakdown of Herwig's epithelial root sheath disturbs tooth root development.

Developmental dynamics : an official publication of the American Association of Anatomists·2023
Same journal

<i>Porphyromonas gingivalis</i>-Induced NETs Mediate Neuroinflammation via TLR4 Activation.

Journal of dental research·2026
Same journal

Oral Burden of Sjögren Disease: A Systematic Review and Meta-analysis.

Journal of dental research·2026
Same journal

Gingival Fibroblast-Driven Osteoimmunology via the IL-33-ILC2-IL-13 Axis.

Journal of dental research·2026
Same journal

Advancing a Global Oral Health Research Agenda.

Journal of dental research·2026
Same journal

YAP/TAZ Drive Oral Leukoplakia Progression and Confer Ferroptosis Vulnerability.

Journal of dental research·2026
Same journal

Multiancestral GWAS of Dental Malocclusion Identifies Multiple Risk Loci.

Journal of dental research·2026
See all related articles

Related Experiment Video

Updated: Jan 14, 2026

The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions
07:34

The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions

Published on: February 16, 2017

8.4K

Notum Regulates Tooth Root Morphogenesis by Modulating Wnt Signaling.

J K Jeong1, H Choi1, T H Kim1

  • 1Cluster for Craniofacial Development and Regeneration Research, Institute of Oral Bioscience, Jeonbuk National University School of Dentistry, Jeonju, Republic of Korea.

Journal of Dental Research
|October 18, 2025
PubMed
Summary
This summary is machine-generated.

Notch signaling is crucial for tooth root development. Loss of Notum, a Wnt antagonist, disrupts molar furcation by altering epithelial-mesenchymal interactions and Wnt pathway activity.

Keywords:
Wnt signaling pathwayWntlessepithelial-mesenchymal interactionsfurcationmolarroot patterning

More Related Videos

Modeling Paracrine Noncanonical Wnt Signaling In Vitro
11:14

Modeling Paracrine Noncanonical Wnt Signaling In Vitro

Published on: December 10, 2021

1.9K
Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients
08:10

Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients

Published on: December 14, 2015

11.8K

Related Experiment Videos

Last Updated: Jan 14, 2026

The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions
07:34

The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions

Published on: February 16, 2017

8.4K
Modeling Paracrine Noncanonical Wnt Signaling In Vitro
11:14

Modeling Paracrine Noncanonical Wnt Signaling In Vitro

Published on: December 10, 2021

1.9K
Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients
08:10

Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients

Published on: December 14, 2015

11.8K

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • Molar root furcation is essential for tooth structure and function.
  • Wnt signaling is vital for tooth development, but its spatial regulation in root formation is unclear.
  • Notum, a secreted Wnt antagonist, has a potential role in regulating tooth morphogenesis.

Purpose of the Study:

  • To investigate the role of Notum in molar root furcation.
  • To elucidate the molecular mechanisms by which Notum regulates root development.
  • To understand the interplay between Wnt signaling and Notum in epithelial-mesenchymal interactions during tooth formation.

Main Methods:

  • Analysis of Notum knockout mice to study furcation defects.
  • Temporospatial expression analysis of Notum during tooth development.
  • Inducible deletion of Wntless (Wls) in dental epithelium to assess Wnt signaling's role.
  • Examination of Hertwig's epithelial root sheath (HERS) extension and mesenchymal signaling.

Main Results:

  • Notum knockout mice showed severe molar root furcation failure and enlarged pulp chambers.
  • Notum deficiency impaired HERS extension and dysregulated mesenchymal Wnt signaling.
  • Loss of Notum led to increased apical pulp cell proliferation and reduced odontoblast differentiation.
  • Epithelial Wnt signaling, mediated by Wls, is required for Notum induction in the mesenchyme.

Conclusions:

  • Notum is a critical regulator of molar root furcation.
  • A feedback loop exists where HERS-derived Wnt signaling induces Notum, which then antagonizes Wnt to promote odontoblast differentiation and furcation.
  • This study reveals novel insights into Wnt-Notum interactions in tooth development.