Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Packed for Ossification: High-Density Bioprinting of hPDC Spheroids in HAMA Toward Endochondral Ossification.

Advanced healthcare materials·2026
Same author

An automated pipeline for tracking and measuring cell spheroids encapsulated in 3D hydrogel systems.

Biofabrication·2026
Same author

The extracellular matrix in peripheral nerve repair and regeneration: a narrative review of its role and therapeutic potential.

Frontiers in neuroanatomy·2025
Same author

Dimethyl Sulfoxide Conditions Induced Pluripotent Stem Cells for more Efficient Nephron Progenitor and Kidney Organoid Differentiation.

Stem cell reviews and reports·2025
Same author

Chitosan-glycerol blended nanofibers for peripheral nerve regeneration applications.

Nanoscale advances·2025
Same author

The Importance of Coating Surface and Composition for Attachment and Survival of Neuronal Cells Under Mechanical Stimulation.

Journal of biomedical materials research. Part A·2025
Same journal

Biomechanical Performance of Zirconia-Calcium Silicate-Silver Hybrid Dental Crown Under Static and Transient Dynamic Loading: A Finite Element and TOPSIS-Based Multi-Criteria Evaluation.

Journal of biomedical materials research. Part B, Applied biomaterials·2026
Same journal

Synthesis, Characterization, and Implantation of Ferulic Acid-Loaded Chitosan/Aloe vera Hydrogels in Rat Full-Thickness Wound Model.

Journal of biomedical materials research. Part B, Applied biomaterials·2026
Same journal

Double-Crosslinked SIS-Derived Collagen/Alginate Hydrogel With Antibacterial and Pro-Regenerative Activities for Soft Tissue Repair.

Journal of biomedical materials research. Part B, Applied biomaterials·2026
Same journal

Silver and Gold Nanoparticles Promote Antimicrobial Activity and Modulate Inflammation in Escherichia coli-Infected Wounds.

Journal of biomedical materials research. Part B, Applied biomaterials·2026
Same journal

Injectable Thermosensitive Placental ECM-Copper Hydrogel With Endothelial Progenitor Cells for Pressure Ulcer Repair.

Journal of biomedical materials research. Part B, Applied biomaterials·2026
Same journal

Strut Thickness and Species-Specific Healing Are Key Considerations in Developing Zinc-Based Biodegradable Arterial Stents.

Journal of biomedical materials research. Part B, Applied biomaterials·2026
See all related articles

Related Experiment Video

Updated: Jan 6, 2026

Fabrication of Size-Controlled and Emulsion-Free Chitosan-Genipin Microgels for Tissue Engineering Applications
05:26

Fabrication of Size-Controlled and Emulsion-Free Chitosan-Genipin Microgels for Tissue Engineering Applications

Published on: April 13, 2022

3.9K

Soft Scaffolds for Nerve Repair: Investigating Glycerol-Plasticized Chitosan Microstructures With In Vitro Complex

Luca Scaccini1,2, Enrique Escarda-Castro3, Adrián Seijas-Gamardo3

  • 1NEST, Scuola Normale Superiore, Pisa, Italy.

Journal of Biomedical Materials Research. Part B, Applied Biomaterials
|October 18, 2025
PubMed
Summary
This summary is machine-generated.

Soft, microgrooved chitosan membranes mimic nerve tissue properties, promoting nerve cell regeneration and alignment. These biomaterials offer a promising platform for peripheral nerve repair and advanced in vitro modeling.

Keywords:
chitosanin vitromicrostructuresnerve modelnerve regenerationscaffolds

More Related Videos

Synthesis of Thermogelling PolyN-isopropylacrylamide-graft-chondroitin Sulfate Composites with Alginate Microparticles for Tissue Engineering
12:22

Synthesis of Thermogelling PolyN-isopropylacrylamide-graft-chondroitin Sulfate Composites with Alginate Microparticles for Tissue Engineering

Published on: October 26, 2016

12.3K
Electrospinning Growth Factor Releasing Microspheres into Fibrous Scaffolds
09:29

Electrospinning Growth Factor Releasing Microspheres into Fibrous Scaffolds

Published on: August 16, 2014

12.7K

Related Experiment Videos

Last Updated: Jan 6, 2026

Fabrication of Size-Controlled and Emulsion-Free Chitosan-Genipin Microgels for Tissue Engineering Applications
05:26

Fabrication of Size-Controlled and Emulsion-Free Chitosan-Genipin Microgels for Tissue Engineering Applications

Published on: April 13, 2022

3.9K
Synthesis of Thermogelling PolyN-isopropylacrylamide-graft-chondroitin Sulfate Composites with Alginate Microparticles for Tissue Engineering
12:22

Synthesis of Thermogelling PolyN-isopropylacrylamide-graft-chondroitin Sulfate Composites with Alginate Microparticles for Tissue Engineering

Published on: October 26, 2016

12.3K
Electrospinning Growth Factor Releasing Microspheres into Fibrous Scaffolds
09:29

Electrospinning Growth Factor Releasing Microspheres into Fibrous Scaffolds

Published on: August 16, 2014

12.7K

Area of Science:

  • Biomaterials Science
  • Neuroscience
  • Tissue Engineering

Background:

  • Peripheral nerve injuries pose significant clinical challenges, often leading to incomplete functional recovery.
  • Current treatments for nerve injuries have limitations, highlighting the need for innovative regenerative strategies.
  • Biomaterial scaffolds mimicking native nerve tissue offer a promising approach to enhance nerve regeneration.

Purpose of the Study:

  • To investigate the regenerative potential of soft, mechanically compliant glycerol-plasticized chitosan (Gly-chi) microstructured membranes.
  • To evaluate the ability of these scaffolds to support glial cell organization and neuronal outgrowth in vitro and ex vivo.
  • To assess the biomimetic properties of Gly-chi membranes for peripheral nerve repair applications.

Main Methods:

  • Fabrication of soft, microgrooved glycerol-plasticized chitosan membranes with nerve-like mechanical properties.
  • In vitro studies using primary Schwann cells (SCs) and human induced pluripotent stem cell (iPSC)-derived sensory neurons.
  • Ex vivo experiments utilizing rat dorsal root ganglia (DRG) explants to assess axonal guidance.

Main Results:

  • Gly-chi membranes promoted Schwann cell invasion, polarization, and alignment.
  • The scaffolds facilitated neurite outgrowth and directional guidance of iPSC-derived sensory neurons when co-cultured with SCs.
  • Ex vivo tests demonstrated that the membranes could orient axonal extension from DRG explants, even over a confluent SC layer.

Conclusions:

  • Glycerol-blended chitosan membranes with physiological stiffness and microgrooves effectively support glial and neuronal organization.
  • These biomimetic scaffolds represent a robust platform for peripheral nerve repair.
  • The findings suggest potential for topographical cues to be transmitted via SC-mediated signaling, offering insights for advanced in vitro modeling.