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Updated: Aug 13, 2026

Anticancer Metal Complexes: Synthesis and Cytotoxicity Evaluation by the MTT Assay
Published on: November 10, 2013
Synthesis, structural characterization, in vitro and in vivo antitumor activities of Pt(II) complexes with
Hai-Qun Zhang1, Nan-Feng Chen1, Hui-Chao Lin1
1Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), Guangxi Key Laboratory of Chemistry and Molecular Engineering of Medicinal Resources, University Engineering Research Center of Distinctive Medicinal Resources Chemistry, Guangxi, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guangxi, China.
Abstract:
Six Pt(II) complexes bearing di(pyridin-2-yl)methanone hydrazone derivative ligands (Pt1 - Pt6) were synthesized and characterized. These complexes exhibited promising antiproliferative activity against multiple cancer cell lines, with low cytotoxicity toward normal human cells. Among them, Pt4 and Pt6 demonstrated the strongest inhibition of A2780 cell proliferation. Mechanistic studies revealed that Pt4 and Pt6 induce apoptosis via mitochondrial dysfunction, accumulation of intracellular Ca2+ and reactive oxygen species, and activation of caspase-3/9. Importantly, Pt4, the most potent agent against A2780 cells, displayed superior antitumor efficacy compared to cisplatin in an A2780 xenograft model, with no significant systemic toxicity.
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