Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Riboswitch-targeted improvement of lysine production in Bacillus and Priestia species.

NPJ science of food·2026
Same author

Observation of tunable chiral spin textures with nonlinear optics.

Nature communications·2026
Same author

Characterization of the virus-host RNA-RNA interactome across important human pathogenic RNA viruses.

PLoS pathogens·2026
Same author

T Cell Receptor Co-Stimulation Through Magnetogenetic Tools: A Platform for Wireless Rewiring of Cellular Signaling.

ACS omega·2026
Same author

Exploring the regulatory potential of RNA structures in 202 cyanobacterial genomes.

Nucleic acids research·2026
Same author

Dextran-based T-cell expansion nanoparticles for manufacturing CAR T cells with augmented efficacy.

Nature communications·2026

Related Experiment Video

Updated: Jan 14, 2026

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles
11:54

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Published on: March 13, 2017

9.8K

Point-of-Care Solid-Phase PCR in a Vertical Microfluidic Chip Integrated with All-Dielectric Nanostructured

Islam Seder1, Leonid Beliaev2, Rodrigo Coronel Téllez1

  • 1Department of Health Technology, Technical University of Denmark, Ørsteds Plads, DK-2800 Kongens Lyngby, Denmark.

ACS Sensors
|October 19, 2025
PubMed
Summary

This study presents a novel microfluidic chip for rapid, sensitive, and cost-effective molecular diagnostics. The integrated nanophotonic enhancement and solid-phase polymerase chain reaction (SP-PCR) enable high-throughput pathogen surveillance at the point of care.

Keywords:
dielectric nanostructurefluid manipulationmultiplexed detectionsolid-phase PCRvertical microfluidic

More Related Videos

Microfluidic Chip Fabrication and Method to Detect Influenza
09:43

Microfluidic Chip Fabrication and Method to Detect Influenza

Published on: March 26, 2013

15.5K
Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays
09:58

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

Published on: June 23, 2022

2.6K

Related Experiment Videos

Last Updated: Jan 14, 2026

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles
11:54

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Published on: March 13, 2017

9.8K
Microfluidic Chip Fabrication and Method to Detect Influenza
09:43

Microfluidic Chip Fabrication and Method to Detect Influenza

Published on: March 26, 2013

15.5K
Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays
09:58

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

Published on: June 23, 2022

2.6K

Area of Science:

  • Biomedical Engineering
  • Molecular Diagnostics
  • Nanophotonics

Background:

  • Multiplexed solid-phase polymerase chain reaction (SP-PCR) is crucial for molecular diagnostics but faces challenges in point-of-care devices.
  • Existing SP-PCR methods suffer from bubble formation, long reaction times, and low signal output.

Purpose of the Study:

  • To engineer a microfluidic chip integrating SP-PCR with nanophotonic enhancement for improved molecular diagnostics.
  • To overcome limitations of traditional SP-PCR for point-of-care applications.

Main Methods:

  • A vertical microfluidic chip design with integrated reagent chambers for automated nucleic acid purification and amplification.
  • Bubble-free, gravity-assisted fluid dynamics and expedited thermal cycling using a dual-heater configuration.
  • Incorporation of an all-dielectric nanostructured metasurface for DNA array immobilization and nanophotonic enhancement.

Main Results:

  • Achieved highly sensitive and high-throughput molecular diagnostics with a detection limit of 10 copies/reaction.
  • Demonstrated bubble-free fluid dynamics and significantly reduced reaction times.
  • Enabled multiplexed detection via guided-mode resonance and SP-PCR.

Conclusions:

  • The developed microfluidic platform offers a scalable, cost-efficient solution for point-of-care diagnostics.
  • Highlights potential for personalized medicine and high-throughput pathogen surveillance.
  • Represents a significant advancement in integrated molecular diagnostic devices.