Related Experiment Video
Updated: Jan 14, 2026
Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
Published on: April 25, 2025
Detection of carbapenemase genes by multiplex recombinase polymerase amplification combined with lateral flow
Tingting Hu1, Siyi Zhou1, Yuqing Xing1
1Department of Clinical Laboratory, the First Affiliated Hospital of Anhui Medical University, Hefei, People's Republic of China; Department of Clinical Laboratory, Anhui Public Health Clinical Center, Hefei, People's Republic of China.
Abstract:
Carbapenem-resistant Enterobacterales (CRE) infections are posing a critical global health threat as their prevalence, multidrug resistance, and mortality rates are all increasing.To address this challenge, we developed a multiplex recombinase polymerase amplification (mRPA) coupled with lateral flow immunoassay strip (LFS) to simultaneously detect five carbapenemase genes (blaKPC, blaNDM, blaOXA-48, blaVIM, and blaIMP) via molecular hybridization. This platform enables rapid, minimal equipments across diverse clinical and community settings.Validation demonstrated no cross-reactivity with non-target clinical strains and a detection limit of 102 CFU/mL (n = 66 clinical samples). Compared to qPCR, the assay achieved 98.2 % sensitivity, 100 % specificity, and 98.5 % concordance. The platform's portability and minimal technical requirements make it suitable for decentralized healthcare settings, including primary hospitals and point-of-care facilities.This multiplex, field-deployable assay provides a critical tool for timely clinical decision-making and precision antimicrobial stewardship in CRE management, particularly in resource-limited regions where conventional molecular diagnostics remain inaccessible.
