Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Drug Metabolism: Phase I Reactions01:17

Drug Metabolism: Phase I Reactions

4.6K
A phase I reaction is a biochemical process that introduces a functionally reactive polar group to a substance. This transformation predominantly occurs in the liver, facilitated by the cytochrome P450 system of hemoproteins situated in the lipophilic endoplasmic reticulum of cells. The metabolite generated through this process can have varying polarities. If it is sufficiently polar, it can be easily excreted in the urine due to its water compatibility. However, if the metabolite is nonpolar,...
4.6K
Diversity of Protists II01:27

Diversity of Protists II

769
Alveolates are a group of organisms recognized by the presence of alveoli, which are cytoplasmic sacs located beneath the cell membrane. While their function remains uncertain, alveoli may help regulate water balance by controlling how much water enters and leaves the cell. In dinoflagellates, these structures may serve as armor plates. There are three major types of alveolates: ciliates, which move using cilia; dinoflagellates, which use flagella for movement; and apicomplexans, which are...
769
Bacterial Phylum Chlamydiae01:29

Bacterial Phylum Chlamydiae

422
The phylum Chlamydiae or Chlamydiota is composed of a single order, Chlamydiales. This phylum consists entirely of obligate intracellular parasites that infect eukaryotic hosts. While human pathogens within this group have been studied extensively, the phylum encompasses many species capable of interacting with various eukaryotic organisms. Members of Chlamydiae are typically small cocci, approximately 0.5 μm in diameter, and exhibit a distinctive developmental cycle. As is characteristic...
422
Cystic Fibrosis: Pathogenesis01:23

Cystic Fibrosis: Pathogenesis

710
Cystic fibrosis (CF), an autosomal recessive disorder, significantly affects the function of exocrine glands. This genetically inherited disease is characterized by the production of thick and sticky mucus, which can severely affect various organs and systems in the body.
CF is primarily caused by a genetic mutation in a chromosome 7 gene coding for the cystic fibrosis transmembrane conductance regulator (CFTR) protein. The most common gene mutation leading to CF is the ΔF508 mutation,...
710
Mutagenicity and Carcinogenicity01:25

Mutagenicity and Carcinogenicity

1.9K
Mutagenicity and carcinogenicity refer to the ability of drugs to cause genetic defects and induce cancer, respectively. The International Agency for Research on Cancer (IARC) classifies agents into four groups based on their carcinogenic potential. Group 1 agents are known human carcinogens; group 2A agents are probably carcinogenic to humans; group 3 agents lack data to support their role in carcinogenesis; and group 4 includes agents for which data support that they are not likely to be...
1.9K
Fungal Phylum Microsporidia01:28

Fungal Phylum Microsporidia

417
Microsporidia are a group of obligate intracellular fungi that were initially classified as protists but were later reclassified based on phylogenetic, molecular, and structural evidence linking them to the Chytridiomycota. These unicellular, non-motile organisms are highly specialized parasites that infect a wide range of animal hosts, including humans. They have evolved extensive genomic and metabolic reductions, making them highly dependent on their hosts for survival.Morphology and Genomic...
417

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Foldify: Web Application for Protein Structure Prediction.

Journal of chemical information and modeling·2026
Same author

C-terminal long-QT type 1 R562S-Kv7.1 variant, the first variant in helix C impairing β-adrenergic response of the slow delayed rectifier K+ channel.

Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology·2026
Same author

PROPTIMUS LIVE: local constrained α-carbon optimization of proteins.

Nucleic acids research·2026
Same author

Dense yet flexible: how ladderanes found in anammox bacteria impact phospholipid membrane structure and properties.

Physical chemistry chemical physics : PCCP·2026
Same author

Atomic Charge Calculator III: a modern platform for calculating partial atomic charges.

Nucleic acids research·2026
Same author

AlphaFind v2: similarity search in AlphaFold DB and TED domains across structural contexts.

Nucleic acids research·2026

Related Experiment Video

Updated: Jan 14, 2026

Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by 32P-postlabeling
09:33

Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by 32P-postlabeling

Published on: March 20, 2018

14.3K

Pathogenicity patterns in cytochrome P450 family.

Anna Špačková1,2, Nina Kadášová1, Ivana Hutařová Vařeková1,3

  • 1Department of Physical Chemistry, Faculty of Science, Palacký University, Olomouc 771 46, Czech Republic.

Bioinformatics Advances
|October 20, 2025
PubMed
Summary

Pathogenicity increases along protein tunnels towards cofactor binding sites in human cytochrome P450s (CYPs). This highlights the critical role of these pathways and cofactor interactions in maintaining enzyme function and drug metabolism.

More Related Videos

Mass Spectrometry and Luminogenic-based Approaches to Characterize Phase I Metabolic Competency of In Vitro Cell Cultures
10:44

Mass Spectrometry and Luminogenic-based Approaches to Characterize Phase I Metabolic Competency of In Vitro Cell Cultures

Published on: March 28, 2017

10.2K
Author Spotlight: Identifying Compensatory Pathways in Malaria Parasites Containing Hypomorphic Allele of Essential Protein Kinases
09:13

Author Spotlight: Identifying Compensatory Pathways in Malaria Parasites Containing Hypomorphic Allele of Essential Protein Kinases

Published on: November 22, 2024

1.8K

Related Experiment Videos

Last Updated: Jan 14, 2026

Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by 32P-postlabeling
09:33

Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by 32P-postlabeling

Published on: March 20, 2018

14.3K
Mass Spectrometry and Luminogenic-based Approaches to Characterize Phase I Metabolic Competency of In Vitro Cell Cultures
10:44

Mass Spectrometry and Luminogenic-based Approaches to Characterize Phase I Metabolic Competency of In Vitro Cell Cultures

Published on: March 28, 2017

10.2K
Author Spotlight: Identifying Compensatory Pathways in Malaria Parasites Containing Hypomorphic Allele of Essential Protein Kinases
09:13

Author Spotlight: Identifying Compensatory Pathways in Malaria Parasites Containing Hypomorphic Allele of Essential Protein Kinases

Published on: November 22, 2024

1.8K

Area of Science:

  • Biochemistry
  • Genetics
  • Computational Biology

Background:

  • Cytochrome P450 proteins are vital for human metabolism, including drug and hormone processing.
  • Known variants often have limited experimental pathogenicity data, necessitating predictive approaches.
  • Computational tools allow for predicting the impact of virtually all possible amino acid mutations.

Purpose of the Study:

  • To comprehensively explore pathogenicity patterns within the human cytochrome P450 protein family.
  • To identify key structural features influencing protein function and variant pathogenicity.
  • To provide insights into disease-associated variants and drug metabolism.

Main Methods:

  • Utilized SIFT, AlphaMissense, and PrimateAI-3D algorithms for pathogenicity analysis.
  • Investigated human cytochrome P450 proteins.
  • Employed MOLE software to identify protein tunnels.

Main Results:

  • Pathogenicity shows a progressive increase along identified protein tunnels towards the cofactor binding site.
  • The integrity of protein tunnels and the cofactor environment is crucial for enzymatic function.
  • Single amino acid changes can disrupt molecular guidance to active sites, impacting protein function.

Conclusions:

  • Structural pathways, particularly protein tunnels, are fundamental for cytochrome P450 functionality.
  • Cofactor interactions are essential for maintaining enzymatic activity.
  • Understanding these patterns aids in interpreting disease-associated variants and predicting drug metabolism efficacy.