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Quantitative Lateral Flow Assay for Meropenem Determination: A Proof-of-Concept Study.

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  • 1Centre for Antimicrobial Optimisation, Imperial College London, London SW7 2AZ, U.K.

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Summary
This summary is machine-generated.

A new lateral flow assay (LFA) enables rapid, point-of-care monitoring of Meropenem antibiotic levels. This innovative method offers a faster alternative to traditional techniques for optimizing critical care patient dosing.

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Pharmacology

Background:

  • Therapeutic drug monitoring (TDM) is crucial for effective antibiotic treatment, especially in critically ill patients.
  • Conventional methods like LC/MS for antibiotic quantification are time-consuming, hindering timely dose adjustments.
  • Rapid and accurate TDM is needed to optimize patient outcomes and reduce antimicrobial resistance.

Purpose of the Study:

  • To adapt a novel competitive lateral flow assay (LFA) for the rapid quantification of Meropenem.
  • To evaluate the performance of the LFA using a beta-lactam receptor protein and a custom image analysis algorithm.
  • To assess the potential of this LFA platform for point-of-care antibiotic monitoring.

Main Methods:

  • Development of a competitive lateral flow assay (LFA) using BlaR-CTD protein and biotinylated BSA-Meropenem-conjugated gold nanoparticles.
  • Adaptation of a dual test line LFA format previously validated for vancomycin.
  • Quantification of Meropenem concentrations using a custom image analysis algorithm to measure test line intensities.

Main Results:

  • The developed LFA demonstrated a concentration-dependent change in test line intensities for Meropenem.
  • A strong correlation (R^2 = 0.9537) was observed between LFA results and Meropenem concentrations.
  • The assay shows promise for rapid, point-of-care antibiotic quantification.

Conclusions:

  • The novel LFA platform offers a potential solution for rapid Meropenem monitoring, overcoming limitations of conventional methods.
  • This technology has the potential for broad application in diverse healthcare settings for point-of-care testing.
  • Further optimization is required for improved accuracy at low Meropenem concentrations, but the proof-of-concept is strong for other beta-lactams.