Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Bioreactor Controls-I

Bioreactor Controls-I

Maintaining optimal conditions within fermenters is essential for maximizing microbial productivity and ensuring process efficiency. This lesson focuses on key parameters—temperature, foam, pH, carbon dioxide, oxygen, and pressure—and their precise measurement and control strategies in fermentation systems.Temperature ControlTemperature regulation is critical due to the exothermic nature of many fermentation processes. In small laboratory fermenters, temperature is commonly monitored using...

iChip

iChip

The cultivation of environmental microorganisms has long been hindered by the inability to replicate complex native conditions in vitro. The isolation chip (iChip) addresses this limitation by facilitating the growth of previously uncultivable microorganisms through in situ incubation. Designed for high-throughput microbial cultivation, the iChip comprises hundreds of microchambers, each capable of housing a single microbial cell. These microchambers are loaded with a mixture of molten agar and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Evaluation of recombinant trypsinogen and chymotrypsinogen production in Komagataella phaffii through co-expression of HAC1 and PDI1.

Microbial cell factories·2026

Same author

Non-agricultural feedstocks for next-generation biomanufacturing with yeasts.

Current opinion in biotechnology·2026

Same author

Rapid quantitative bioanalysis using LC-HRIM-QTOFMS with flexible sample delivery and variable traveling wave profiles.

Analytica chimica acta·2026

Same author

Bioconversion of the C1 substrate methanol to succinic acid with engineered Komagataella phaffii.

Bioresource technology·2026

Same author

Rewiring methanol metabolism in Komagataella phaffii through implementation and evolution of a heterologous RuMP cycle.

Metabolic engineering·2026

Same author

Polydiacetylene (PDA) coated paper-based fluorescence sensor for the detection and quantification of bisphenol.

Lab on a chip·2026

Search research articles

Related Experiment Video

Updated: Jun 8, 2026

Chip-based Three-dimensional Cell Culture in Perfused Micro-bioreactors

Chip-based Three-dimensional Cell Culture in Perfused Micro-bioreactors

Published on: May 21, 2008

Online Monitoring of Chip-Based Microscale Perfusion Fermentations.

Sabrina M Cramer^1,2, Shubham Gurav^1,3, David Glinsner^1,3

¹acib - austrian centre of industrial biotechnology, Muthgasse 11, Vienna 1190, Austria.

|October 20, 2025

Summary

A novel online analytical platform enables real-time monitoring of microbioreactor fermentations. This system allows for efficient screening and development of bioprocesses, improving strain productivity and product yield.

More Related Videos

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation

Published on: December 6, 2013

Multi-Stream Perfusion Bioreactor Integrated with Outlet Fractionation for Dynamic Cell Culture

Multi-Stream Perfusion Bioreactor Integrated with Outlet Fractionation for Dynamic Cell Culture

Published on: July 20, 2022

Related Experiment Videos

Last Updated: Jun 8, 2026

Chip-based Three-dimensional Cell Culture in Perfused Micro-bioreactors

Chip-based Three-dimensional Cell Culture in Perfused Micro-bioreactors

Published on: May 21, 2008

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation

Published on: December 6, 2013

Multi-Stream Perfusion Bioreactor Integrated with Outlet Fractionation for Dynamic Cell Culture

Multi-Stream Perfusion Bioreactor Integrated with Outlet Fractionation for Dynamic Cell Culture

Published on: July 20, 2022

Area of Science:

Biotechnology
Analytical Chemistry
Bioprocess Engineering

Background:

Conventional offline sampling in biomanufacturing provides limited feedback on fermentation parameters.
Bench-scale bioprocess development requires improved methods for real-time monitoring.

Purpose of the Study:

To develop a sensitive and selective online analytical platform for monitoring chip-based microbioreactors.
To enable real-time analysis of fermentation bioprocess parameters and metabolites.

Main Methods:

A platform was constructed using commercially available components: valves, 2DLC hardware, LC separation, and online tandem mass spectrometry.
Microbioreactors (20 μL) of *Saccharomyces cerevisiae* were cultivated with low-flow perfusion rates.
A multiplexed configuration with a stream selection valve and a microscale-adapted 2DLC-MS/MS system was employed.

Main Results:

The platform successfully monitored fermentations of lactic-acid-producing *S. cerevisiae* over 5-24 hours.
Online measurements of metabolites from parallel fermentations were achieved without blockages or cross-contamination.
The system demonstrated suitability for online monitoring of product quantity and key metabolites.

Conclusions:

The developed platform offers real-time monitoring for fermentation biotechnology, enabling rapid strain selection and screening.
Minimal consumption of biological material and media (<1.5 mL/24h) makes the platform highly efficient.
The adaptable platform can be used for monitoring diverse biomolecules and targeting other biotechnology products.