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Updated: Jan 14, 2026

A Fast Air-dry Dropping Chromosome Preparation Method Suitable for FISH in Plants
Published on: December 16, 2015
A chromosome-scale assembly of Ormosia boluoensis (Fabaceae)
Jin-Pi Zhu1, Zheng-Feng Wang2,3,4,5, Fang Cheng1
1Management Office of Guangdong Xiangtoushan National Natural Reserve, Huizhou, 516166, China.
None:
The genus Ormosia is in the Fabaceae family. China is one of its centers of origin, with almost all species being endemic, suggesting the need for genomic studies to understand its evolution, conservation, and use. Therefore, we generated a chromosome-scale genome assembly of O. boluoensis using Oxford Nanopore long reads combined with Illumina short reads. The initial genome assembly generated by NextDenovo had a total length of 1,638,935,015 bp and a contig N50 of 16,933,043 bp. The assembly after Hi-C reads scaffolding was 1,565,663,440 bp with the scaffold N50 of 201,058,012 bp, and 1,561,022,207 bp (99.70%) for sequences were anchored to 8 chromosomes. Repeats accounted for 75.06% of the assembly. There were 51,822 predicted genes. The BUSCO evaluation showed 98.2% completeness at genome assessment level. The high-quality assembly and annotation provide valuable insights into the genomic characteristics of O. boluoensis, providing resources for its effective conservation and future utilization.
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