Enzyme-assisted structural characterization of ulvan from Ulva pertusa by hydrophilic interaction liquid
Chunying Du1, Xinyu Wang1, Leke Qiao2
1College of Food Science and Engineering, Ocean University of China, Qingdao, 266404, China.
Abstract:
Ulvan, a sulfated polysaccharide from green algae, shows diverse bioactivities with potential in functional foods and pharmaceuticals. Structure characterization is crucial for elucidating its active mechanism. In this study, ulvan from Ulva pertusa was selectively depolymerized using PL25 ulvan lyase, and the resulting ulva oligosaccharides (UO) were analyzed by hydrophilic interaction liquid chromatography-mass spectrometry (HILIC-MS) before and after NaBH4 reduction. HILIC-MS and HILIC-ESI-MS/MS identified five major even-numbered oligosaccharides, including ΔUA-Rha3S (45 %), ΔUA-Rha3S-Xyl-Rha3S (33 %), ΔUA-Rha3S-Xyl2S-Rha3S (2 %), ΔUA-Rha3S-Xyl-Rha3S-Xyl-Rha3S (11 %), and ΔUA-Rha3S-GlcA-Rha3S-Xyl2S-Rha3S (8 %). Based on HILIC-ESI-MS/MS and nuclear magnetic resonance analyses, the backbone of ulvan was mainly composed of β-D-GlcA-(1 → 4)-α-L-Rha3S and β-D-Xyl-(1 → 4)-α-L-Rha3S, in an approximate ratio of 4.6:1, with ~15 % sulfated Xyl residues. These results provided a basis for further exploration of the structure-function relationships of ulvan.
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