riboCIRC v2.0: an expanded resource for translatable CircRNAs

Area of Science:

• Genomics and Bioinformatics
• Molecular Biology
• Proteomics

Background:

• Circular RNAs (circRNAs) are increasingly recognized for their regulatory roles.
• The translatome, encompassing all translated RNAs, is crucial for understanding cellular function.
• Characterizing translatable circRNAs and their encoded peptides is essential for functional genomics.

Purpose of the Study:

• To present riboCIRC v2.0, a significantly updated database for translatable circRNAs and their encoded peptides.
• To enhance research into circRNA translation and function characterization.
• To provide a comprehensive resource for diverse biological systems.

Main Methods:

• Expanded data collection of translatable circRNAs and finite ORF-encoded peptides.
• Integrated new evidence layers for circRNA translation, including RNA editing and RBP interactions.
• Performed systematic in silico annotation of encoded peptides for properties and functions.

Main Results:

• riboCIRC v2.0 documents 5212 predicted translatable circRNAs and 7563 predicted peptides.
• Enhanced evidence integration includes ribosome profiling, mass spectrometry, IRES, m6A, RNA editing, and RBP interactions.
• Comprehensive peptide annotation details physicochemical properties, structures, and potential functions.

Conclusions:

• riboCIRC v2.0 offers substantially expanded content and enhanced functionality.
• The updated database serves as a powerful resource for investigating translatable circRNAs and their products.
• Improved data exploration and visualization facilitate research in diverse biological systems.