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Updated: Jan 6, 2026
Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
Published on: March 21, 2018
Electrochemical aptasensor based on Chrysanthemum indicum L.-derived carbon materials for detection of
Jutarat Sonjai1, Pakin Noppawan1,2, Nidchakarn Theamwong3
1Department of Chemistry and Centre of Excellence for Innovation in Chemistry, Faculty of Science, Mahasarakham University, Maha Sarakham, 44150, Thailand. Jantima.u@msu.ac.th.
Abstract:
A novel, eco-friendly, and label-free electrochemical aptasensor was successfully developed for the detection of carcinoembryonic antigen (CEA). Screen-printed carbon electrodes (SPCEs) were fabricated and modified with gold nanoparticles and activated carbon derived from Chrysanthemum indicum L. flowers, a waste byproduct from beverage production. The activated carbon surface was further functionalized with carboxylic acid groups to enhance the number of aptamer binding sites. The modified electrode exhibited a significantly improved electrochemical response toward potassium ferri-ferrocyanide compared to the unmodified SPCE. Upon immobilization of a CEA-specific aptamer under optimized conditions, the sensor demonstrated a linear detection range of 25-1000 ng mL-1 for CEA. The aptasensor also showed excellent analytical performances, with high selectivity, stability, and reproducibility, along with satisfactory recovery in spiked serum samples (92.65% to 117.18%). Compared to conventional detection methods, this sensor offers key advantages, including reduced cost, rapid response, and ease of fabrication and operation.
