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Updated: Jan 14, 2026
Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA
Published on: May 2, 2018
Air sampling and analysis method to determine worker exposure levels to N-isopropyl-N'-phenyl-p-phenylenediamine
Tomiko Tashiro1, Akito Takeuchi1,2, Kenta Ishii3,4
1Kinki Osaka Safety and Health Service Center, Japan Industrial Safety and Health Association, 2-3-8 Tosabori, Nishi-Ku, Osaka 550-0001, Japan.
Objectives:
This study aimed to develop a method for determining workers' exposure concentrations to airborne N-isopropyl-N'-phenyl-p-phenylenediamine (IPPD).
Methods:
A hydrophobic polytetrafluoroethylene (HPB PTFE) membrane filter was used as the sampling medium. IPPD was extracted from the HPB PTFE filter with acetonitrile, and its concentration in the extracted solution was determined using a high-performance liquid chromatograph equipped with a photodiode array detector. The validating method was performed using the following parameters: extraction and retention efficiency, storage stability, method quantitation limit, and reproducibility.
Results:
The IPPD extraction efficiency from the spiked HPB PTFE filters was 96%-100%. The IPPD retention efficiencies on the HPB PTFE filters were 72%-99%, with relative standard deviations indicating the overall reproducibility of 0.9%-3.1%. IPPD on the HPB PTFE filter remained stable for at least 7 days at 4°C. The method quantitation limit was 12.5 μg/sample.
Conclusions:
We successfully developed a method to measure workers' exposure to airborne IPPD within the concentration range of 0.05-4 mg/m3, which will assist risk assessments.
