Scope and Synthetic Applications of the Aryl-Alcohol Oxidase from Streptomyces hiroshimensis (ShAAO)
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Updated: Jan 14, 2026

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
Published on: April 1, 2016
Christian Ascaso-Alegre1, Paula Cinca-Fernando2, Tom L Roberts3
1Institute of Chemical Synthesis and Homogeneous Catalysis (ISQCH, Spanish National Research Council (CSIC)-University of Zaragoza, Pedro Cerbuna 12, 50009 Zaragoza, Spain.
Abstract:
The sustainable production of carbonyl compounds is of growing interest due to their broad academic and industrial interest. Herein, we explored the aryl-alcohol oxidase from Streptomyces hiroshimensis (ShAAO), mapping its activity across diverse alcohols, revealing a distinct profile from other natural AAOs. Integrated into tandem organocatalytic processes, ShAAO enabled the synthesis of various chiral compounds, including an intermediate for paroxetine. Gram-scale reactions delivered high yields and turnover numbers exceeding 65000. Immobilization further enhanced stability, highlighting ShAAO as an efficient and industrially relevant biocatalyst.
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