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Author Spotlight: Improving Reproducibility in Vascular Organoids Using ROCK Inhibitors and Microwell Confinement

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Angiogenesis induction using organoid-tissue modules: A platform for modular vessel construction.

Jin Ju Park^1,2, Eunjeong Seo^1,2, HyeRan Gwak^1,2

¹Department of Dental Regenerative Biotechnology, School of Dentistry, Seoul National University, Republic of Korea.

Journal of Tissue Engineering

|October 22, 2025

Summary

This summary is machine-generated.

Researchers developed vascularized organoid tissue modules (Angio-TMs) using endothelial cells. These modules show improved vascularization and potential for regenerative medicine applications.

Keywords:

angiogenesis human adipose-derived mesenchymal stem cell human umbilical vein endothelial cell organoid-tissue module transforming growth factor-beta

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Area of Science:

Tissue Engineering
Regenerative Medicine
Vascular Biology

Background:

Angiogenesis is critical for tissue regeneration but challenging in organoid systems.
Functional vasculature is a key limitation in current organoid-based therapies.
Developing vascularized constructs is vital for advancing regenerative medicine.

Purpose of the Study:

To engineer vascularized organoid tissue modules (Angio-TMs).
To assess the vascular functionality and stability of Angio-TMs.
To investigate methods for enhancing angiogenic potential in engineered tissues.

Main Methods:

Incorporation of human umbilical vein endothelial cells (HUVECs) into scaffold-free organoid constructs.
Generation of Angio-TMs with 1% HUVECs for structural stability and reproducibility.
In vitro and in vivo assessment of endothelial differentiation and vascular functionality.
Inhibition of transforming growth factor (TGF)-β signaling to modulate angiogenic potential.

Main Results:

Developed highly reproducible and structurally stable Angio-TMs.
Demonstrated clear endothelial differentiation and vascular functionality in vitro and in vivo.
Achieved a 2.5-fold increase in vessel length density upon TGF-β signaling inhibition.
Confirmed Angio-TMs as a robust platform for vascularized tissue engineering.

Conclusions:

Angio-TMs represent a promising modular platform for engineering vascularized tissues.
The developed Angio-TMs exhibit significant potential for regenerative medicine and tissue transplantation.
Modulating TGF-β signaling offers a strategy to enhance angiogenic capacity in engineered tissues.