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Nanomechanics of Drug-target Interactions and Antibacterial Resistance Detection
Published on: October 25, 2013
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Combining aptamers for thiamphenicol and chloramphenicol for detecting both antibiotics
Yun Shu1,2, Shiyuan Liu2, Juewen Liu2
1School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, 225005, China.
Abstract
None:
A DNA aptamer named TAP-1 for thiamphenicol (TAP) was selected using the capture-systematic evolution of ligands by exponential enrichment (SELEX) technique, exhibiting a dissociation constant (Kd) of 6.4 μM as determined by isothermal titration calorimetry (ITC). When combined with a previously selected chloramphenicol (CAP) aptamer, they collectively can differentiate TAP and CAP.
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