Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Mouse Models of Cancer Study

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Detection of Gene Fusions in Soft Tissue Sarcoma Using Next-Generation Sequencing.

Genes·2026

Same author

Chronic radiation-induced dermatitis: what is new in 2024? A narrative review.

Reports of practical oncology and radiotherapy : journal of Greatpoland Cancer Center in Poznan and Polish Society of Radiation Oncology·2026

Same author

Long-term efficacy of radical treatment of patients with clear cell sarcoma.

European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·2026

Same author

Metabolomic Study of 7-Ethyl-9-(<i>N</i>-methyl)aminomethyl-10-hydroxycamptothecin Derivative (NMe)-The Chemotherapeutic Drug Candidate Versus Irinotecan (IR) on a Mouse Model.

Metabolites·2026

Same author

Chemotherapy in dedifferentiated chondrosarcoma: From neoadjuvant to palliative treatment options.

Critical reviews in oncology/hematology·2026

Same author

Integrated Immune and Molecular Profiling Identifies Prognostic Subgroups and Therapeutic Targets in Chondrosarcoma.

International journal of molecular sciences·2026

Search research articles

Related Experiment Video

Updated: Jan 6, 2026

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models

Published on: October 28, 2021

Genetically Modified Mouse Models for Sarcoma Research: A Comprehensive Review.

Piotr Remiszewski^1,2, Eryk Siedlecki^1,2, Marlena Wełniak-Kamińska³

¹Department of Soft Tissue/Bone Sarcoma and Melanoma, Maria Sklodowska- Curie National Research Institute of Oncology, Warsaw, Poland.

Current Oncology Reports

|October 22, 2025

Summary

Genetically engineered mouse models (GEMMs) precisely model sarcoma genetics, aiding research into these rare cancers. While valuable, improving GEMM accuracy for advanced disease and immunotherapy is crucial for better preclinical insights.

Keywords:

Animal models CRISPR-Cas9 Cre-loxP Genetically engineered mouse Preclinical model Soft tissue sarcoma

More Related Videos

A Mouse Model of Incompletely Resected Soft Tissue Sarcoma for Testing Neoadjuvant Therapies

A Mouse Model of Incompletely Resected Soft Tissue Sarcoma for Testing Neoadjuvant Therapies

Published on: July 28, 2020

Modeling Primary Bone Tumors and Bone Metastasis with Solid Tumor Graft Implantation into Bone

Modeling Primary Bone Tumors and Bone Metastasis with Solid Tumor Graft Implantation into Bone

Published on: September 9, 2020

Related Experiment Videos

Last Updated: Jan 6, 2026

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models

Published on: October 28, 2021

A Mouse Model of Incompletely Resected Soft Tissue Sarcoma for Testing Neoadjuvant Therapies

A Mouse Model of Incompletely Resected Soft Tissue Sarcoma for Testing Neoadjuvant Therapies

Published on: July 28, 2020

Modeling Primary Bone Tumors and Bone Metastasis with Solid Tumor Graft Implantation into Bone

Modeling Primary Bone Tumors and Bone Metastasis with Solid Tumor Graft Implantation into Bone

Published on: September 9, 2020

Area of Science:

Oncology
Genetics
Preclinical Research

Background:

Sarcomas are rare, heterogeneous mesenchymal tumours with poor prognoses, necessitating novel therapeutic strategies.
Effective preclinical models are vital but challenging due to sarcoma complexity and limited resources.

Purpose of the Study:

To review the utility and limitations of genetically engineered mouse models (GEMMs) for sarcoma research.
To highlight advancements in GEMM technology for modeling sarcoma initiation, progression, and treatment response.

Main Methods:

Utilizing Cre-loxP and CRISPR-Cas9 systems for precise genetic manipulation in mice.
Developing GEMMs that recapitulate sarcoma histopathology, cell of origin, and tumor-immune interactions.

Main Results:

GEMMs accurately model recurrent sarcoma genetics, including oncogene activation and tumor suppressor loss.
These models reproduce key features of human sarcomas, enabling studies on tumor initiation, radiotherapy, and immunotherapy.
Limitations include breeding burden, genetic variability, off-target effects, and underrepresentation of genomic complexity, impacting predictive value for advanced disease.

Conclusions:

GEMMs are essential for investigating sarcoma mechanisms and therapy response, offering mechanistic fidelity.
Improving GEMM translational relevance requires integrating Cre-loxP with CRISPR-Cas9, standardizing protocols, and aligning models with specific sarcoma subtypes.