Rationally Trapped Polycrystalline Perovskite in Lanthanide MOF Cages for Ammonia-Mediated Nucleic Acid Intelligent...

Area of Science:

• Materials Science
• Nanotechnology
• Chemical Engineering

Background:

• Perovskite nanoparticles (PNPs) are promising for fluorescence biosensing but suffer from poor stability in polar solvents.
• Metal-organic frameworks (MOFs), especially lanthanide MOFs (Ln-MOFs), offer enhanced stability and tunable properties for material integration.

Purpose of the Study:

• To develop a stable hybrid material by integrating PNPs into Ln-MOFs for improved fluorescence biosensing.
• To elucidate the mechanism behind the enhanced physical and fluorescence properties of these hybrid materials.
• To create a sensitive and specific biosensing platform for on-site nucleic acid detection.

Main Methods:

• Theoretical calculations identified 2,2'-bipyridine-5,5'-dicarboxylic acid as a ligand for Eu3+ sensitization and Pb2+ chemisorption.
• Hierarchical Eu-MOFs were constructed using 4-bromobutyric acid for in situ perovskite growth.
• Zwitterionic ligands were generated via S_N2 reaction for stable PNP dispersion in polar solvents.

Main Results:

• The hybrid PNPs@Ln-MOF demonstrated a 50-fold increase in fluorescence lifetime and enhanced stability in polar solvents.
• A highly sensitive fluorescence color shift in response to ammonia was observed.
• The developed device achieved precise on-site nucleic acid assay with a limit of detection (LOD) of 200 fM, utilizing CRISPR/Cas technology.

Conclusions:

• Integrating PNPs into Ln-MOFs effectively addresses stability issues in polar solvents.
• The hybrid material offers a generalizable strategy for ammonia-mediated biosensing.
• This work pioneers advanced applications of perovskite-based hybrid materials in sensitive, on-site biosensing.